Abstract
Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12502-12509 |
| Number of pages | 8 |
| Journal | Optics Express |
| Volume | 17 |
| Issue number | 15 |
| DOIs | |
| State | Published - Jul 20 2009 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation. / Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M G; Matthews, Dennis L; Demos, Stavros G.
In: Optics Express, Vol. 17, No. 15, 20.07.2009, p. 12502-12509.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
AU - Lin, Bevin
AU - Urayama, Shiro
AU - Saroufeem, Ramez M G
AU - Matthews, Dennis L
AU - Demos, Stavros G.
PY - 2009/7/20
Y1 - 2009/7/20
N2 - Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation.
AB - Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation.
UR - http://www.scopus.com/inward/record.url?scp=67749118117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67749118117&partnerID=8YFLogxK
U2 - 10.1364/OE.17.012502
DO - 10.1364/OE.17.012502
M3 - Article
C2 - 19654651
AN - SCOPUS:67749118117
VL - 17
SP - 12502
EP - 12509
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 15
ER -