Real-time measurements of milk fat globule membrane modulation during simulated intestinal digestion using electron paramagnetic resonance spectroscopy

Maha Alshehab, Madhu S. Budamagunta, John C. Voss, Nitin Nitin

Research output: Contribution to journalArticle

Abstract

Milk Fat Globules with their unique interfacial structure and membrane composition are a key nutritional source for mammalian infants, however, there is a limited understanding of the dynamics of fat digestion in these structures. Lipid digestion is an interfacial process involving interactions of enzymes and bile salts with the interface of suspended lipid droplets in an aqueous environment. In this study, we have developed an electron paramagnetic resonance spectroscopy approach to evaluate real time dynamics of milk fat globules interfacial structure during simulated intestinal digestion. To measure these dynamics, natural milk fat globule membrane was labeled with EPR-active probe, partitioning of EPR probes into MFGs membrane was validated using saturation-recovery measurements and calculation of the depth parameter Φ. After validation, the selected spin probe was used to evaluate the membrane's fluidity as a measure of the interface's modulation in the presence of bile salts and pancreatic lipase. Independently, bile salts were found to have a rigidifying effect on the spin probed MFGM, while pancreatic lipase resulted in an increase in membrane fluidity. When combined, the effect of lipase appears to be diminished in the presence of bile salts. These results indicate the efficacy of EPR in providing an insight into small time scale molecular dynamics of phospholipid interfaces in milk fat globules. Understanding interfacial dynamics of naturally occurring complex structures can significantly aid in understanding the role of interfacial composition and structural complexity in delivery of nutrients during digestion.

Original languageEnglish (US)
Article number110511
JournalColloids and Surfaces B: Biointerfaces
Volume184
DOIs
StatePublished - Dec 1 2019

Fingerprint

milk
globules
Electron Spin Resonance Spectroscopy
fats
Oils and fats
Time measurement
Bile Acids and Salts
Paramagnetic resonance
Digestion
Spectrum Analysis
electron paramagnetic resonance
Lipase
time measurement
Modulation
Spectroscopy
membranes
Membranes
Lipases
modulation
Membrane Fluidity

Keywords

  • Bile salts
  • Digestion
  • Electron paramagnetic resonance
  • Milk fat globules
  • Milk fat globules membrane
  • Pancreatic lipase

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Real-time measurements of milk fat globule membrane modulation during simulated intestinal digestion using electron paramagnetic resonance spectroscopy. / Alshehab, Maha; Budamagunta, Madhu S.; Voss, John C.; Nitin, Nitin.

In: Colloids and Surfaces B: Biointerfaces, Vol. 184, 110511, 01.12.2019.

Research output: Contribution to journalArticle

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