Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmami, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6091
DOIs
StatePublished - 2006
EventOptical Biopsy VI - San Jose, CA, United States
Duration: Jan 24 2006Jan 24 2006

Other

OtherOptical Biopsy VI
CountryUnited States
CitySan Jose, CA
Period1/24/061/24/06

Fingerprint

Tissue
Imaging techniques
Microstructure
Microscopes
Operating rooms
Image acquisition
Optical devices
Light scattering
Animals
Lighting
Polarization
Wavelength

Keywords

  • Autofluorescence
  • Microscopy
  • Real-time imaging
  • Tissue microstructures
  • Tissue optical properties

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Lin, B., Lieber, C. A., Fitzgerald, J. T., Michalopoulou, A. P., Raman, R. N., Pivetti, C. D., ... Demos, S. G. (2006). Real-time imaging of tissue microstructures using intrinsic optical signatures. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6091). [60910G] https://doi.org/10.1117/12.644954

Real-time imaging of tissue microstructures using intrinsic optical signatures. / Lin, Bevin; Lieber, Chad A.; Fitzgerald, Jason T.; Michalopoulou, Andromachi P.; Raman, Rajesh N.; Pivetti, Christopher D.; Troppmami, Christoph; Matthews, Dennis L; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091 2006. 60910G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lin, B, Lieber, CA, Fitzgerald, JT, Michalopoulou, AP, Raman, RN, Pivetti, CD, Troppmami, C, Matthews, DL & Demos, SG 2006, Real-time imaging of tissue microstructures using intrinsic optical signatures. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6091, 60910G, Optical Biopsy VI, San Jose, CA, United States, 1/24/06. https://doi.org/10.1117/12.644954
Lin B, Lieber CA, Fitzgerald JT, Michalopoulou AP, Raman RN, Pivetti CD et al. Real-time imaging of tissue microstructures using intrinsic optical signatures. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091. 2006. 60910G https://doi.org/10.1117/12.644954
Lin, Bevin ; Lieber, Chad A. ; Fitzgerald, Jason T. ; Michalopoulou, Andromachi P. ; Raman, Rajesh N. ; Pivetti, Christopher D. ; Troppmami, Christoph ; Matthews, Dennis L ; Demos, Stavros G. / Real-time imaging of tissue microstructures using intrinsic optical signatures. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091 2006.
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