Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmami, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2006
EventOptical Biopsy VI - San Jose, CA, United States
Duration: Jan 24 2006Jan 24 2006


OtherOptical Biopsy VI
Country/TerritoryUnited States
CitySan Jose, CA


  • Autofluorescence
  • Microscopy
  • Real-time imaging
  • Tissue microstructures
  • Tissue optical properties

ASJC Scopus subject areas

  • Engineering(all)


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