Abstract
We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6091 |
| DOIs | |
| State | Published - 2006 |
| Event | Optical Biopsy VI - San Jose, CA, United States Duration: Jan 24 2006 → Jan 24 2006 |
Other
| Other | Optical Biopsy VI |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/24/06 → 1/24/06 |
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Keywords
- Autofluorescence
- Microscopy
- Real-time imaging
- Tissue microstructures
- Tissue optical properties
ASJC Scopus subject areas
- Engineering(all)
Cite this
Real-time imaging of tissue microstructures using intrinsic optical signatures. / Lin, Bevin; Lieber, Chad A.; Fitzgerald, Jason T.; Michalopoulou, Andromachi P.; Raman, Rajesh N.; Pivetti, Christopher D.; Troppmami, Christoph; Matthews, Dennis L; Demos, Stavros G.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091 2006. 60910G.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Real-time imaging of tissue microstructures using intrinsic optical signatures
AU - Lin, Bevin
AU - Lieber, Chad A.
AU - Fitzgerald, Jason T.
AU - Michalopoulou, Andromachi P.
AU - Raman, Rajesh N.
AU - Pivetti, Christopher D.
AU - Troppmami, Christoph
AU - Matthews, Dennis L
AU - Demos, Stavros G.
PY - 2006
Y1 - 2006
N2 - We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.
AB - We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.
KW - Autofluorescence
KW - Microscopy
KW - Real-time imaging
KW - Tissue microstructures
KW - Tissue optical properties
UR - http://www.scopus.com/inward/record.url?scp=33646202097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646202097&partnerID=8YFLogxK
U2 - 10.1117/12.644954
DO - 10.1117/12.644954
M3 - Conference contribution
AN - SCOPUS:33646202097
SN - 0819461334
SN - 9780819461339
VL - 6091
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -