Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin; Chad A. Lieber; Jason T. Fitzgerald; Andromachi P. Michalopoulou; Rajesh N. Raman; Christopher D. Pivetti; Christoph Troppmami; Dennis L Matthews; Stavros G. Demos

doi:10.1117/12.644954

Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmami, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6091
DOIs	https://doi.org/10.1117/12.644954
State	Published - 2006
Event	Optical Biopsy VI - San Jose, CA, United States Duration: Jan 24 2006 → Jan 24 2006

Other

Other	Optical Biopsy VI
Country	United States
City	San Jose, CA
Period	1/24/06 → 1/24/06

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Keywords

Autofluorescence
Microscopy
Real-time imaging
Tissue microstructures
Tissue optical properties

ASJC Scopus subject areas

Engineering(all)

Cite this

Lin, B., Lieber, C. A., Fitzgerald, J. T., Michalopoulou, A. P., Raman, R. N., Pivetti, C. D., Troppmami, C., Matthews, D. L., & Demos, S. G. (2006). Real-time imaging of tissue microstructures using intrinsic optical signatures. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6091). [60910G] https://doi.org/10.1117/12.644954

Real-time imaging of tissue microstructures using intrinsic optical signatures. / Lin, Bevin; Lieber, Chad A.; Fitzgerald, Jason T.; Michalopoulou, Andromachi P.; Raman, Rajesh N.; Pivetti, Christopher D.; Troppmami, Christoph; Matthews, Dennis L; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091 2006. 60910G.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lin, B, Lieber, CA, Fitzgerald, JT, Michalopoulou, AP, Raman, RN, Pivetti, CD, Troppmami, C, Matthews, DL & Demos, SG 2006, Real-time imaging of tissue microstructures using intrinsic optical signatures. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6091, 60910G, Optical Biopsy VI, San Jose, CA, United States, 1/24/06. https://doi.org/10.1117/12.644954

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AB - We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

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Access to Document

10.1117/12.644954