Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin; Chad A. Lieber; Jason T. Fitzgerald; Andromachi P. Michalopoulou; Rajesh N. Raman; Christopher D. Pivetti; Christoph Troppmami; Dennis L Matthews; Stavros G. Demos

doi:10.1117/12.644954

Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmami, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6091
DOIs	https://doi.org/10.1117/12.644954
State	Published - 2006
Event	Optical Biopsy VI - San Jose, CA, United States Duration: Jan 24 2006 → Jan 24 2006

Other

Other	Optical Biopsy VI
Country	United States
City	San Jose, CA
Period	1/24/06 → 1/24/06

Keywords

Autofluorescence
Microscopy
Real-time imaging
Tissue microstructures
Tissue optical properties

ASJC Scopus subject areas

Engineering(all)

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10.1117/12.644954

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Real-time imaging of tissue microstructures using intrinsic optical signatures. / Lin, Bevin; Lieber, Chad A.; Fitzgerald, Jason T.; Michalopoulou, Andromachi P.; Raman, Rajesh N.; Pivetti, Christopher D.; Troppmami, Christoph; Matthews, Dennis L; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6091 2006. 60910G.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lin, B, Lieber, CA, Fitzgerald, JT, Michalopoulou, AP, Raman, RN, Pivetti, CD, Troppmami, C, Matthews, DL & Demos, SG 2006, Real-time imaging of tissue microstructures using intrinsic optical signatures. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6091, 60910G, Optical Biopsy VI, San Jose, CA, United States, 1/24/06. https://doi.org/10.1117/12.644954

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title = "Real-time imaging of tissue microstructures using intrinsic optical signatures",

abstract = "We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.",

keywords = "Autofluorescence, Microscopy, Real-time imaging, Tissue microstructures, Tissue optical properties",

author = "Bevin Lin and Lieber, {Chad A.} and Fitzgerald, {Jason T.} and Michalopoulou, {Andromachi P.} and Raman, {Rajesh N.} and Pivetti, {Christopher D.} and Christoph Troppmami and Matthews, {Dennis L} and Demos, {Stavros G.}",

year = "2006",

doi = "10.1117/12.644954",

language = "English (US)",

isbn = "0819461334",

volume = "6091",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

note = "Optical Biopsy VI ; Conference date: 24-01-2006 Through 24-01-2006",

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T1 - Real-time imaging of tissue microstructures using intrinsic optical signatures

AU - Lin, Bevin

AU - Lieber, Chad A.

AU - Fitzgerald, Jason T.

AU - Michalopoulou, Andromachi P.

AU - Raman, Rajesh N.

AU - Pivetti, Christopher D.

AU - Troppmami, Christoph

AU - Matthews, Dennis L

AU - Demos, Stavros G.

PY - 2006

Y1 - 2006

N2 - We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

AB - We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

KW - Autofluorescence

KW - Microscopy

KW - Real-time imaging

KW - Tissue microstructures

KW - Tissue optical properties

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M3 - Conference contribution

AN - SCOPUS:33646202097

SN - 0819461334

SN - 9780819461339

VL - 6091

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

T2 - Optical Biopsy VI

Y2 - 24 January 2006 through 24 January 2006

ER -

Real-time imaging of tissue microstructures using intrinsic optical signatures

Abstract

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Keywords

ASJC Scopus subject areas

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