Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy

Yuliang Zhang, Ramya H. Tunuguntla, Pyung On Choi, Aleksandr Noy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In-plane mobility of proteins in lipid membranes is one of the fundamental mechanisms supporting biological functionality. Here we use high-speed atomic force microscopy (HS-AFM) to show that a novel type of biomimetic channel-carbon nanotube porins (CNTPs)-is also laterally mobile in supported lipid membranes, mimicking biological protein behaviour. HS-AFM can capture real-time dynamics of CNTP motion in the supported lipid bilayer membrane, build long-term trajectories of the CNTP motion and determine the diffusion coefficients associated with this motion. Our analysis shows that diffusion coefficients of CNTPs fall into the same range as those of proteins in supported lipid membranes. CNTPs in HS-AFM experiments often exhibit ‘directed’ diffusion behaviour, which is common for proteins in live cell membranes. This article is part of the themed issue ‘Membrane pores: from structure and assembly, to medicine and technology’.

Original languageEnglish (US)
Article number20160226
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume372
Issue number1726
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

carbon nanotubes
porins
Porins
Carbon Nanotubes
atomic force microscopy
Atomic Force Microscopy
Membrane Lipids
Atomic force microscopy
lipids
diffusivity
Proteins
proteins
Biological membranes
biomimetics
Biomimetics
lipid bilayers
Lipid bilayers
Membranes
Lipid Bilayers
Cell membranes

Keywords

  • 2D diffusion
  • CNTPs
  • HS-AFM
  • Supported lipid bilayer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy. / Zhang, Yuliang; Tunuguntla, Ramya H.; Choi, Pyung On; Noy, Aleksandr.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, No. 1726, 20160226, 01.01.2017.

Research output: Contribution to journalArticle

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