Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells

Dongqing Zhang, Tingyang Zhou, Feng He, Yi Rong, Shin Hee Lee, Shiyong Wu, Li Zuo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ionizing radiation (IR) in cancer radiotherapy can induce damage to neighboring cells via non-targeted effects by irradiated cells. These so-called bystander effects remain an area of interest as it may provide enhanced efficacy in killing carcinomas with minimal radiation. It is well known that reactive oxygen species (ROS) are ubiquitous among most biological activities. However, the role of ROS in bystander effects has not been thoroughly elucidated. We hypothesized that gradient irradiation (GI) has enhanced therapeutic effects via the ROS-mediated bystander pathways as compared to uniform irradiation (UI). We evaluated ROS generation, viability, and apoptosis in breast cancer cells (MCF-7) exposed to UI (5 Gy) or GI (8-2 Gy) in radiation fields at 2, 24 and 48 h after IR. We found that extracellular ROS release induced by GI was higher than that by UI at both 24 h (p < 0.001) and 48 h (p < 0.001). More apoptosis and less viability were observed in GI when compared to UI at either 24 h or 48 h after irradiation. The mean effective doses (ED) of GI were ~130% (24 h) and ~48% (48 h) higher than that of UI, respectively. Our results suggest that GI is superior to UI regarding redox mechanisms, ED, and toxic dosage to surrounding tissues.

Original languageEnglish (US)
Pages (from-to)41622-41636
Number of pages15
JournalOncotarget
Volume7
Issue number27
DOIs
StatePublished - Jul 5 2016
Externally publishedYes

Fingerprint

Bystander Effect
Reactive Oxygen Species
Breast Neoplasms
Ionizing Radiation
Apoptosis
Radiation Dosage
Poisons
MCF-7 Cells
Therapeutic Uses
Oxidation-Reduction
Radiotherapy
Radiation
Carcinoma
Neoplasms

Keywords

  • breast cancer cells
  • bystander effects
  • gradient irradiation
  • MCF-7
  • reactive oxygen species

ASJC Scopus subject areas

  • Oncology

Cite this

Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells. / Zhang, Dongqing; Zhou, Tingyang; He, Feng; Rong, Yi; Lee, Shin Hee; Wu, Shiyong; Zuo, Li.

In: Oncotarget, Vol. 7, No. 27, 05.07.2016, p. 41622-41636.

Research output: Contribution to journalArticle

Zhang, Dongqing ; Zhou, Tingyang ; He, Feng ; Rong, Yi ; Lee, Shin Hee ; Wu, Shiyong ; Zuo, Li. / Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells. In: Oncotarget. 2016 ; Vol. 7, No. 27. pp. 41622-41636.
@article{6d7d80fc1340448499b9d866d9ed2e2e,
title = "Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells",
abstract = "Ionizing radiation (IR) in cancer radiotherapy can induce damage to neighboring cells via non-targeted effects by irradiated cells. These so-called bystander effects remain an area of interest as it may provide enhanced efficacy in killing carcinomas with minimal radiation. It is well known that reactive oxygen species (ROS) are ubiquitous among most biological activities. However, the role of ROS in bystander effects has not been thoroughly elucidated. We hypothesized that gradient irradiation (GI) has enhanced therapeutic effects via the ROS-mediated bystander pathways as compared to uniform irradiation (UI). We evaluated ROS generation, viability, and apoptosis in breast cancer cells (MCF-7) exposed to UI (5 Gy) or GI (8-2 Gy) in radiation fields at 2, 24 and 48 h after IR. We found that extracellular ROS release induced by GI was higher than that by UI at both 24 h (p < 0.001) and 48 h (p < 0.001). More apoptosis and less viability were observed in GI when compared to UI at either 24 h or 48 h after irradiation. The mean effective doses (ED) of GI were ~130{\%} (24 h) and ~48{\%} (48 h) higher than that of UI, respectively. Our results suggest that GI is superior to UI regarding redox mechanisms, ED, and toxic dosage to surrounding tissues.",
keywords = "breast cancer cells, bystander effects, gradient irradiation, MCF-7, reactive oxygen species",
author = "Dongqing Zhang and Tingyang Zhou and Feng He and Yi Rong and Lee, {Shin Hee} and Shiyong Wu and Li Zuo",
year = "2016",
month = "7",
day = "5",
doi = "10.18632/oncotarget.9517",
language = "English (US)",
volume = "7",
pages = "41622--41636",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "27",

}

TY - JOUR

T1 - Reactive oxygen species formation and bystander effects in gradient irradiation on human breast cancer cells

AU - Zhang, Dongqing

AU - Zhou, Tingyang

AU - He, Feng

AU - Rong, Yi

AU - Lee, Shin Hee

AU - Wu, Shiyong

AU - Zuo, Li

PY - 2016/7/5

Y1 - 2016/7/5

N2 - Ionizing radiation (IR) in cancer radiotherapy can induce damage to neighboring cells via non-targeted effects by irradiated cells. These so-called bystander effects remain an area of interest as it may provide enhanced efficacy in killing carcinomas with minimal radiation. It is well known that reactive oxygen species (ROS) are ubiquitous among most biological activities. However, the role of ROS in bystander effects has not been thoroughly elucidated. We hypothesized that gradient irradiation (GI) has enhanced therapeutic effects via the ROS-mediated bystander pathways as compared to uniform irradiation (UI). We evaluated ROS generation, viability, and apoptosis in breast cancer cells (MCF-7) exposed to UI (5 Gy) or GI (8-2 Gy) in radiation fields at 2, 24 and 48 h after IR. We found that extracellular ROS release induced by GI was higher than that by UI at both 24 h (p < 0.001) and 48 h (p < 0.001). More apoptosis and less viability were observed in GI when compared to UI at either 24 h or 48 h after irradiation. The mean effective doses (ED) of GI were ~130% (24 h) and ~48% (48 h) higher than that of UI, respectively. Our results suggest that GI is superior to UI regarding redox mechanisms, ED, and toxic dosage to surrounding tissues.

AB - Ionizing radiation (IR) in cancer radiotherapy can induce damage to neighboring cells via non-targeted effects by irradiated cells. These so-called bystander effects remain an area of interest as it may provide enhanced efficacy in killing carcinomas with minimal radiation. It is well known that reactive oxygen species (ROS) are ubiquitous among most biological activities. However, the role of ROS in bystander effects has not been thoroughly elucidated. We hypothesized that gradient irradiation (GI) has enhanced therapeutic effects via the ROS-mediated bystander pathways as compared to uniform irradiation (UI). We evaluated ROS generation, viability, and apoptosis in breast cancer cells (MCF-7) exposed to UI (5 Gy) or GI (8-2 Gy) in radiation fields at 2, 24 and 48 h after IR. We found that extracellular ROS release induced by GI was higher than that by UI at both 24 h (p < 0.001) and 48 h (p < 0.001). More apoptosis and less viability were observed in GI when compared to UI at either 24 h or 48 h after irradiation. The mean effective doses (ED) of GI were ~130% (24 h) and ~48% (48 h) higher than that of UI, respectively. Our results suggest that GI is superior to UI regarding redox mechanisms, ED, and toxic dosage to surrounding tissues.

KW - breast cancer cells

KW - bystander effects

KW - gradient irradiation

KW - MCF-7

KW - reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=84992156227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992156227&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.9517

DO - 10.18632/oncotarget.9517

M3 - Article

VL - 7

SP - 41622

EP - 41636

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 27

ER -