Reactive oxygen species are involved in BMP-induced dendritic growth in cultured rat sympathetic neurons

Vidya Chandrasekaran, Charlotte Lea, Jose Carlo Sosa, Dennis Higgins, Pamela J Lein

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previous studies have shown that bone morphogenetic proteins (BMPs) promote dendritic growth in sympathetic neurons; however, the downstream signaling molecules that mediate the dendrite promoting activity of BMPs are not well characterized. Here we test the hypothesis that reactive oxygen species (ROS)-mediated signaling links BMP receptor activation to dendritic growth. In cultured rat sympathetic neurons, exposure to any of the three mechanistically distinct antioxidants, diphenylene iodinium (DPI), nordihydroguaiaretic acid (NGA) or desferroxamine (DFO), blocked de novo BMP-induced dendritic growth. Addition of DPI to cultures previously induced with BMP to extend dendrites caused dendritic retraction while DFO and NGA prevented further growth of dendrites. The inhibition of the dendrite promoting activity of BMPs by antioxidants was concentration-dependent and occurred without altering axonal growth or neuronal cell survival. Antioxidant treatment did not block BMP activation of SMAD 1,5 as determined by nuclear localization of these SMADs. While BMP treatment did not cause a detectable increase in intracellular ROS in cultured sympathetic neurons as assessed using fluorescent indicator dyes, BMP treatment increased the oxygen consumption rate in cultured sympathetic neurons as determined using the Seahorse XF24 Analyzer, suggesting increased mitochondrial activity. In addition, BMPs upregulated expression of NADPH oxidase 2 (NOX2) and either pharmacological inhibition or siRNA knockdown of NOX2 significantly decreased BMP-7 induced dendritic growth. Collectively, these data support the hypothesis that ROS are involved in the downstream signaling events that mediate BMP7-induced dendritic growth in sympathetic neurons, and suggest that ROS-mediated signaling positively modulates dendritic complexity in peripheral neurons.

Original languageEnglish (US)
Pages (from-to)116-125
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume67
DOIs
StatePublished - Jul 1 2015

Fingerprint

Bone Morphogenetic Proteins
Reactive Oxygen Species
Neurons
Growth
Dendrites
Masoprocol
Deferoxamine
Antioxidants
NADPH Oxidase
Bone Morphogenetic Protein Receptors
Bone Morphogenetic Protein 7
Smegmamorpha
Fluorescent Dyes
Oxygen Consumption
Small Interfering RNA
Cell Survival
Pharmacology

Keywords

  • Antioxidants
  • Bone morphogenetic proteins
  • Dendrites
  • Free radicals
  • Reactive oxygen species
  • Sympathetic neurons

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Reactive oxygen species are involved in BMP-induced dendritic growth in cultured rat sympathetic neurons. / Chandrasekaran, Vidya; Lea, Charlotte; Sosa, Jose Carlo; Higgins, Dennis; Lein, Pamela J.

In: Molecular and Cellular Neuroscience, Vol. 67, 01.07.2015, p. 116-125.

Research output: Contribution to journalArticle

Chandrasekaran, Vidya ; Lea, Charlotte ; Sosa, Jose Carlo ; Higgins, Dennis ; Lein, Pamela J. / Reactive oxygen species are involved in BMP-induced dendritic growth in cultured rat sympathetic neurons. In: Molecular and Cellular Neuroscience. 2015 ; Vol. 67. pp. 116-125.
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