Re-entry into the cell cycle is required for bFGF-induced oligodendroglial dedifferentiation and survival

Judith B. Grinspan, Matthew F. Reeves, Markella J. Coulaloglou, Daniel Nathanson, David E Pleasure

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Remyelination in the CNS following demyelinating disease may be accomplished by surviving mature oligodendrocytes that dedifferentiate, proliferate, migrate, and finally regenerate myelin. We previously reported that basic fibroblast growth factor (bFGF) induces oligodendrocytes in primary mixed glial cultures to dedifferentiate and synthesize DNA (Grinspan et al.: J Neurosci Res 36:672-680, 1993). We now show that this effect is direct and not mediated through the effects of bFGF on other cell types, because we were able to demonstrate similar changes in oligodendrocyte phenotype in enriched oligodendrocyte cultures prepared by immunopanning. The bFGF-induced reversion to the precursor stage of the oligodendroglial lineage can be blocked by agents that inhibit entry to the cell cycle; thus oligodendroglial dedifferentiation is dependent on proliferation. We also report that 2 days of bFGF treatment inhibits oligodendroglial apoptosis. However, when oligodendroglia are prevented from entering the cell cycle in the presence of bFGF, apoptotic cell death is increased. Thus, bFGF induces oligodendroglial dedifferentiation if oligodendroglial DNA synthesis can occur but causes oligodendoglial apoptosis when oligodendroglial DNA synthesis is prevented.

Original languageEnglish (US)
Pages (from-to)456-464
Number of pages9
JournalJournal of Neuroscience Research
Volume46
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

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Fibroblast Growth Factor 2
Oligodendroglia
Cell Cycle
DNA
Apoptosis
Demyelinating Diseases
Myelin Sheath
Neuroglia
Cell Death
Phenotype

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Re-entry into the cell cycle is required for bFGF-induced oligodendroglial dedifferentiation and survival. / Grinspan, Judith B.; Reeves, Matthew F.; Coulaloglou, Markella J.; Nathanson, Daniel; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 46, No. 4, 1996, p. 456-464.

Research output: Contribution to journalArticle

Grinspan, Judith B. ; Reeves, Matthew F. ; Coulaloglou, Markella J. ; Nathanson, Daniel ; Pleasure, David E. / Re-entry into the cell cycle is required for bFGF-induced oligodendroglial dedifferentiation and survival. In: Journal of Neuroscience Research. 1996 ; Vol. 46, No. 4. pp. 456-464.
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