Re-electrospraying splash-landed proteins and nanoparticles

W. Henry Benner, Gregory S. Lewis, Susanne V. Hering, Brent Selgelke, Michelle Corzett, James E. Evans, Felice C Lightstone

Research output: Contribution to journalArticlepeer-review


FITC-albumin, Lsr-F, or fluorescent polystyrene latex particles were electrosprayed from aqueous buffer and subjected to dispersion by differential electrical mobility at atmospheric pressure. A resulting narrow size cut of singly charged molecular ions or particles was passed through a condensation growth tube collector to create a flow stream of small water droplets, each carrying a single ion or particle. The droplets were splash landed (impacted) onto a solid or liquid temperature controlled surface. Small pools of droplets containing size-selected particles, FITC-albumin, or Lsr-F were recovered, re-electrosprayed, and, when analyzed a second time by differential electrical mobility, showed increased homogeneity. Transmission electron microscopy (TEM) analysis of the size-selected Lsr-F sample corroborated the mobility observation.

Original languageEnglish (US)
Pages (from-to)2498-2504
Number of pages7
JournalAnalytical Chemistry
Issue number5
StatePublished - Mar 6 2012
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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