Re-electrospraying splash-landed proteins and nanoparticles

W. Henry Benner, Gregory S. Lewis, Susanne V. Hering, Brent Selgelke, Michelle Corzett, James E. Evans, Felice C Lightstone

Research output: Contribution to journalArticle

Abstract

FITC-albumin, Lsr-F, or fluorescent polystyrene latex particles were electrosprayed from aqueous buffer and subjected to dispersion by differential electrical mobility at atmospheric pressure. A resulting narrow size cut of singly charged molecular ions or particles was passed through a condensation growth tube collector to create a flow stream of small water droplets, each carrying a single ion or particle. The droplets were splash landed (impacted) onto a solid or liquid temperature controlled surface. Small pools of droplets containing size-selected particles, FITC-albumin, or Lsr-F were recovered, re-electrosprayed, and, when analyzed a second time by differential electrical mobility, showed increased homogeneity. Transmission electron microscopy (TEM) analysis of the size-selected Lsr-F sample corroborated the mobility observation.

Original languageEnglish (US)
Pages (from-to)2498-2504
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number5
DOIs
StatePublished - Mar 6 2012
Externally publishedYes

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Nanoparticles
Ions
Proteins
Atmospheric pressure
Condensation
Buffers
Particle size
Transmission electron microscopy
Water
Liquids
Temperature
FITC-albumin
styrofoam

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Benner, W. H., Lewis, G. S., Hering, S. V., Selgelke, B., Corzett, M., Evans, J. E., & Lightstone, F. C. (2012). Re-electrospraying splash-landed proteins and nanoparticles. Analytical Chemistry, 84(5), 2498-2504. https://doi.org/10.1021/ac203298w

Re-electrospraying splash-landed proteins and nanoparticles. / Benner, W. Henry; Lewis, Gregory S.; Hering, Susanne V.; Selgelke, Brent; Corzett, Michelle; Evans, James E.; Lightstone, Felice C.

In: Analytical Chemistry, Vol. 84, No. 5, 06.03.2012, p. 2498-2504.

Research output: Contribution to journalArticle

Benner, WH, Lewis, GS, Hering, SV, Selgelke, B, Corzett, M, Evans, JE & Lightstone, FC 2012, 'Re-electrospraying splash-landed proteins and nanoparticles', Analytical Chemistry, vol. 84, no. 5, pp. 2498-2504. https://doi.org/10.1021/ac203298w
Benner WH, Lewis GS, Hering SV, Selgelke B, Corzett M, Evans JE et al. Re-electrospraying splash-landed proteins and nanoparticles. Analytical Chemistry. 2012 Mar 6;84(5):2498-2504. https://doi.org/10.1021/ac203298w
Benner, W. Henry ; Lewis, Gregory S. ; Hering, Susanne V. ; Selgelke, Brent ; Corzett, Michelle ; Evans, James E. ; Lightstone, Felice C. / Re-electrospraying splash-landed proteins and nanoparticles. In: Analytical Chemistry. 2012 ; Vol. 84, No. 5. pp. 2498-2504.
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