Rational design and application of responsive α-helical peptide hydrogels

Eleanor F. Banwell, Edgardo S. Abelardo, Dave J. Adams, Martin A. Birchall, Adam Corrigan, Athene M. Donald, Mark Kirkland, Louise C. Serpell, Michael F. Butler, Derek N. Woolfson

Research output: Contribution to journalArticle

333 Scopus citations

Abstract

Biocompatible hydrogels have a wide variety of potential applications in biotechnology and medicine, such as the controlled delivery and release of cells, cosmetics and drugs, and as supports for cell growth and tissue engineering. Rational peptide design and engineering are emerging as promising new routes to such functional biomaterials. Here, we present the first examples of rationally designed and fully characterized self-assembling hydrogels based on standard linear peptides with purely α-helical structures, which we call hydrogelating self-assembling fibres (hSAFs). These form spanning networks of α-helical fibrils that interact to give self-supporting physical hydrogels of >99 water content. The peptide sequences can be engineered to alter the underlying mechanism of gelation and, consequently, the hydrogel properties. Interestingly, for example, those with hydrogen-bonded networks of fibrils melt on heating, whereas those formed through hydrophobic fibril-fibril interactions strengthen when warmed. The hSAFs are dual-peptide systems that gel only on mixing, which gives tight control over assembly. These properties raise possibilities for using the hSAFs as substrates in cell culture. We have tested this in comparison with the widely used Matrigel substrate, and demonstrate that, like Matrigel, hSAFs support both growth and differentiation of rat adrenal pheochromocytoma cells for sustained periods in culture.

Original languageEnglish (US)
Pages (from-to)596-600
Number of pages5
JournalNature Materials
Volume8
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

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ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics
  • Materials Science(all)
  • Chemistry(all)

Cite this

Banwell, E. F., Abelardo, E. S., Adams, D. J., Birchall, M. A., Corrigan, A., Donald, A. M., Kirkland, M., Serpell, L. C., Butler, M. F., & Woolfson, D. N. (2009). Rational design and application of responsive α-helical peptide hydrogels. Nature Materials, 8(7), 596-600. https://doi.org/10.1038/nmat2479