Rates of oxygen consumption of fertilized and unfertilized Asterias, Arbacia, and Spisula eggs

Barbara A Horwitz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The rates of oxygen consumption of unfertilized and fertilized eggs of Arbacia punctulata, Asterias forbesii, and Spisula solidissima were determined at 15 °C using an oxygen electrode. Unfertilized eggs of Spisula have the lowest rate of oxygen uptake while Asterias ova have the highest, those of Arbacia being intermediate in value. Fertilization of Arbacia and Asterias eggs is accompanied by an immediate, but transient increase in the rate of oxygen uptake which lasts approximately 1.5 min and is significantly greater in the eggs of Arbacia than in those of Asterias. Within 3 min after insemination, the oxygen uptake of fertilized Asterias eggs returns to a rate comparable to that of the unfertilized eggs; the rate of oxygen consumption of Arbacia eggs also falls, but to a level which is still significantly higher than that of the unfertilized ova. This transient increase, which is suggested to be associated with the elevation of the fertilization membrane, could not be observed in the ova of Spisula because the methods necessary for obtaining successful fertilization did not allow measurements to be made earlier than 6 min following insemination.

Original languageEnglish (US)
Pages (from-to)620-625
Number of pages6
JournalExperimental Cell Research
Volume38
Issue number3
DOIs
StatePublished - Jan 1 1965
Externally publishedYes

Fingerprint

Spisula
Arbacia
Asterias
Oxygen Consumption
Eggs
Ovum
Fertilization
Oxygen
Insemination
Zygote
Electrodes
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rates of oxygen consumption of fertilized and unfertilized Asterias, Arbacia, and Spisula eggs. / Horwitz, Barbara A.

In: Experimental Cell Research, Vol. 38, No. 3, 01.01.1965, p. 620-625.

Research output: Contribution to journalArticle

@article{3803ce89bf834a70bfe5f615c9d2c456,
title = "Rates of oxygen consumption of fertilized and unfertilized Asterias, Arbacia, and Spisula eggs",
abstract = "The rates of oxygen consumption of unfertilized and fertilized eggs of Arbacia punctulata, Asterias forbesii, and Spisula solidissima were determined at 15 °C using an oxygen electrode. Unfertilized eggs of Spisula have the lowest rate of oxygen uptake while Asterias ova have the highest, those of Arbacia being intermediate in value. Fertilization of Arbacia and Asterias eggs is accompanied by an immediate, but transient increase in the rate of oxygen uptake which lasts approximately 1.5 min and is significantly greater in the eggs of Arbacia than in those of Asterias. Within 3 min after insemination, the oxygen uptake of fertilized Asterias eggs returns to a rate comparable to that of the unfertilized eggs; the rate of oxygen consumption of Arbacia eggs also falls, but to a level which is still significantly higher than that of the unfertilized ova. This transient increase, which is suggested to be associated with the elevation of the fertilization membrane, could not be observed in the ova of Spisula because the methods necessary for obtaining successful fertilization did not allow measurements to be made earlier than 6 min following insemination.",
author = "Horwitz, {Barbara A}",
year = "1965",
month = "1",
day = "1",
doi = "10.1016/0014-4827(65)90385-X",
language = "English (US)",
volume = "38",
pages = "620--625",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Rates of oxygen consumption of fertilized and unfertilized Asterias, Arbacia, and Spisula eggs

AU - Horwitz, Barbara A

PY - 1965/1/1

Y1 - 1965/1/1

N2 - The rates of oxygen consumption of unfertilized and fertilized eggs of Arbacia punctulata, Asterias forbesii, and Spisula solidissima were determined at 15 °C using an oxygen electrode. Unfertilized eggs of Spisula have the lowest rate of oxygen uptake while Asterias ova have the highest, those of Arbacia being intermediate in value. Fertilization of Arbacia and Asterias eggs is accompanied by an immediate, but transient increase in the rate of oxygen uptake which lasts approximately 1.5 min and is significantly greater in the eggs of Arbacia than in those of Asterias. Within 3 min after insemination, the oxygen uptake of fertilized Asterias eggs returns to a rate comparable to that of the unfertilized eggs; the rate of oxygen consumption of Arbacia eggs also falls, but to a level which is still significantly higher than that of the unfertilized ova. This transient increase, which is suggested to be associated with the elevation of the fertilization membrane, could not be observed in the ova of Spisula because the methods necessary for obtaining successful fertilization did not allow measurements to be made earlier than 6 min following insemination.

AB - The rates of oxygen consumption of unfertilized and fertilized eggs of Arbacia punctulata, Asterias forbesii, and Spisula solidissima were determined at 15 °C using an oxygen electrode. Unfertilized eggs of Spisula have the lowest rate of oxygen uptake while Asterias ova have the highest, those of Arbacia being intermediate in value. Fertilization of Arbacia and Asterias eggs is accompanied by an immediate, but transient increase in the rate of oxygen uptake which lasts approximately 1.5 min and is significantly greater in the eggs of Arbacia than in those of Asterias. Within 3 min after insemination, the oxygen uptake of fertilized Asterias eggs returns to a rate comparable to that of the unfertilized eggs; the rate of oxygen consumption of Arbacia eggs also falls, but to a level which is still significantly higher than that of the unfertilized ova. This transient increase, which is suggested to be associated with the elevation of the fertilization membrane, could not be observed in the ova of Spisula because the methods necessary for obtaining successful fertilization did not allow measurements to be made earlier than 6 min following insemination.

UR - http://www.scopus.com/inward/record.url?scp=50549212054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50549212054&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(65)90385-X

DO - 10.1016/0014-4827(65)90385-X

M3 - Article

VL - 38

SP - 620

EP - 625

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 3

ER -