Rat embryos cultured under copper-deficient conditions develop abnormally and are characterized by an impaired oxidant defense system

Susan N. Hawk, Janet Y. Uriu-Hare, George P. Daston, Margaret A. Jankowski, Catherine Kwik-Uribe, Robert B. Rucker, Carl L Keen

Research output: Contribution to journalArticle

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Abstract

Rat embryos (gestation days 9.0 and 10.0) obtained from dams that were fed a Cu-adequate (8 μg Cu/g) or Cu-deficient (< 0.5 μg Cu/g) diet were cultured for 48 hr in Cu-adequate (16.2 μM) or Cu-deficient (1.0 μM) rat serum. Control embryos cultured in control serum were morphologically normal. Embryos from Cu-deficient dams developed abnormally when cultured in Cu-deficient serum; the abnormalities included distended hindbrains, blisters, blood pooling, and cardiac defects. Control embryos cultured in Cu-deficient serum and Cu-deficient embryos cultured in control serum also showed abnormal development, but to a lesser degree than that of the Cu-deficient embryos cultured in Cu-deficient serum. To test the idea that the above abnormalities were due in part to free radical-induced damage occurring secondary to an impaired oxidant defense system, a chemiluminescence assay was used to detect superoxide dismutase (SOD) activity in the cultured embryos. SOD activity was lowest in embryos cultured in Cu-deficient serum. When the Cu-deficient serum was supplemented with antioxidants (CuZnSOD or glutathione peroxidase), its teratogenicity was reduced. These data support the idea that an impaired oxidant defense system contributes to the dysmorphology associated with Cu deficiency. However, the Cu-deficient embryos also had low cytochrome c oxidase activity compared to control embryos - thus, multiple factors are likely contributing to Cu deficiency-induced abnormalities.

Original languageEnglish (US)
Pages (from-to)310-320
Number of pages11
JournalTeratology
Volume57
Issue number6
DOIs
StatePublished - Jun 1998

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Oxidants
Rats
Copper
Embryonic Structures
Serum
Dams
Superoxide Dismutase
Chemiluminescence
Electron Transport Complex IV
Nutrition
Glutathione Peroxidase
Free Radicals
Assays
Blood
Antioxidants
Rhombencephalon
Defects
Blister
Luminescence
Diet

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Rat embryos cultured under copper-deficient conditions develop abnormally and are characterized by an impaired oxidant defense system. / Hawk, Susan N.; Uriu-Hare, Janet Y.; Daston, George P.; Jankowski, Margaret A.; Kwik-Uribe, Catherine; Rucker, Robert B.; Keen, Carl L.

In: Teratology, Vol. 57, No. 6, 06.1998, p. 310-320.

Research output: Contribution to journalArticle

Hawk, Susan N. ; Uriu-Hare, Janet Y. ; Daston, George P. ; Jankowski, Margaret A. ; Kwik-Uribe, Catherine ; Rucker, Robert B. ; Keen, Carl L. / Rat embryos cultured under copper-deficient conditions develop abnormally and are characterized by an impaired oxidant defense system. In: Teratology. 1998 ; Vol. 57, No. 6. pp. 310-320.
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