RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs

Julius Ebinu, Drell A. Bottorff, Edmond Y.W. Chan, Stacey L. Stang, Robert J. Dunn, James C. Stone

Research output: Contribution to journalArticle

508 Citations (Scopus)

Abstract

RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of 'EF hands' that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling and changes in cell morphology. Signaling was associated with partitioning of RasGRP protein into the membrane fraction. Sustained ligand-induced signaling and membrane partitioning were absent when the DAG-binding domain was deleted. RasGRP is expressed in the nervous system, where it may couple changes in DAG and possibly calcium concentrations to Ras activation.

Original languageEnglish (US)
Pages (from-to)1082-1086
Number of pages5
JournalScience
Volume280
Issue number5366
DOIs
StatePublished - May 15 1998
Externally publishedYes

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Diglycerides
Nucleotides
Calcium
Proteins
EF Hand Motifs
Guanosine
Nervous System
Catalytic Domain
Membrane Proteins
Fibroblasts
Ligands
Membranes

ASJC Scopus subject areas

  • General

Cite this

Ebinu, J., Bottorff, D. A., Chan, E. Y. W., Stang, S. L., Dunn, R. J., & Stone, J. C. (1998). RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. Science, 280(5366), 1082-1086. https://doi.org/10.1126/science.280.5366.1082

RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. / Ebinu, Julius; Bottorff, Drell A.; Chan, Edmond Y.W.; Stang, Stacey L.; Dunn, Robert J.; Stone, James C.

In: Science, Vol. 280, No. 5366, 15.05.1998, p. 1082-1086.

Research output: Contribution to journalArticle

Ebinu, J, Bottorff, DA, Chan, EYW, Stang, SL, Dunn, RJ & Stone, JC 1998, 'RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs', Science, vol. 280, no. 5366, pp. 1082-1086. https://doi.org/10.1126/science.280.5366.1082
Ebinu, Julius ; Bottorff, Drell A. ; Chan, Edmond Y.W. ; Stang, Stacey L. ; Dunn, Robert J. ; Stone, James C. / RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. In: Science. 1998 ; Vol. 280, No. 5366. pp. 1082-1086.
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