Rapid recruitment of NMDA receptor transport packets to nascent synapses

Philip Washbourne, Jennie E. Bennett, A Kimberley Usrey

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

Although many of the molecules involved in synaptogenesis have been identified, the sequence and kinetics of synapse assembly in the central nervous system (CNS) remain largely unknown. We used simultaneous time-lapse imaging of fluorescent glutamate receptor subunits and presynaptic proteins in rat cortical neurons in vitro to determine the dynamics and time course of N-methyl-D-aspartate receptor (NMDAR) recruitment to nascent synapses. We found that both NMDA and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) subunits are present in mobile transport packets in neurons before and during synaptogenesis. NMDAR transport packets are more mobile than AMPAR subunits, moving along microtubules at about 4 μm/min, and are recruited to sites of axodendritic contact within minutes. Whereas NMDAR recruitment to new synapses can be either concurrent with or independent of the protein PSD-95, AMPARs are recruited with a slower time course. Thus, glutamatergic synapses can form rapidly by the sequential delivery of modular transport packets containing glutamate receptors.

Original languageEnglish (US)
Pages (from-to)751-759
Number of pages9
JournalNature Neuroscience
Volume5
Issue number8
DOIs
StatePublished - 2002

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N-Methyl-D-Aspartate Receptors
Synapses
Glutamate Receptors
Time-Lapse Imaging
Neurons
Protein Subunits
N-Methylaspartate
Microtubules
Central Nervous System
Acids
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rapid recruitment of NMDA receptor transport packets to nascent synapses. / Washbourne, Philip; Bennett, Jennie E.; Usrey, A Kimberley.

In: Nature Neuroscience, Vol. 5, No. 8, 2002, p. 751-759.

Research output: Contribution to journalArticle

Washbourne, Philip ; Bennett, Jennie E. ; Usrey, A Kimberley. / Rapid recruitment of NMDA receptor transport packets to nascent synapses. In: Nature Neuroscience. 2002 ; Vol. 5, No. 8. pp. 751-759.
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