Rapid quantitative immunohistochemical assessment of human peripheral neuropathies using a monoclonal antibody against nerve growth factor receptor

E. Scarpini, S. Beretta, A. H. Ross, M. Moggio, S. Jann, David E Pleasure, G. Scarlato

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

An analysis of nerve growth factor (NGF) receptor expression and density in human sural nerve biopsies was performed by immunocytochemistry with a murine monoclonal antibody against the human NGF receptor. Quantitative assessment of immunostaining density was made by histospectrophotometry on frozen sections. Although there was enhanced expression of NGF receptor within endoneurium in all patients with clinical neuropathies, expression was highest in nerves with axonal disease, consistent with the proposal that disruption of axon-Schwann cell interactions triggers the reexpression of the NGF receptor. These results with human nerves, together with previous studies with animal models, suggest that NGF and NGF receptor play important roles in the general response to neuronal injury.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalJournal of Neurology
Volume236
Issue number8
DOIs
StatePublished - Dec 1989
Externally publishedYes

Keywords

  • Immunocytochemistry
  • Monoclonal antibody
  • Nerve growth factor receptor
  • Peripheral neuropathy
  • Schwann cell

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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