Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology

Frank Chuang, Dora M. Gutierrez, Christine P. Nguyen, David C. Johnson, Richard A. Palmer, James B. Richards, John T Chang, Steven R. Visuri, Bill W. Colston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In response to a broad-based need for point-of-care multiplex diagnostic capability, we have developed a novel hybrid platform to analyze optically encoded microspheres arranged on a 2-dimensional planar array. The microspheres which we have initially selected are developed by Luminex Inc. as substrates for sandwich-type fluorescent immunoassays and are typically used in conjunction with a customized flow analyzer. CCD-based optics are the essential feature which enables the development of a rugged diagnostic instrument which can be scaled for point-of-care applications. We have characterized the Multiplex Immunoassay Diagnostic System (MIDS) using a benchtop prototype built around a conventional 12-bit CCD. This system is capable of resolving up to 6 discrete classes of fluorescent microbeads, and measuring their corresponding reporter signal. The MIDS sensitivity to the phycoerythrin (PE) reporter compared favorably to that of the reference Luminex flow system, and is capable of identifying viral, bacterial, and protein simulants in laboratory samples, at concentrations less than 1μg/ml. The ability to resolve small differences in the average PE fluorescence is a direct function of CCD performance, and may be a necessary trade-off for developing a portable and economical detection system. However, we are confident that the MIDS platform can easily be scaled to meet the nominal requirements of any given point-of-care or screening application, and furthermore provide much-needed diagnostic functionality in this particular environment.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsT. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn
Pages22-26
Number of pages5
Volume4958
DOIs
StatePublished - 2003
Externally publishedYes
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States
Duration: Jan 26 2003Jan 28 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems
CountryUnited States
CitySan Jose, CA
Period1/26/031/28/03

Fingerprint

immunoassay
Microarrays
Charge coupled devices
Microspheres
charge coupled devices
platforms
Optics
Screening
Fluorescence
Proteins
analyzers
screening
prototypes
optics
Substrates
proteins
fluorescence
requirements
sensitivity

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Chuang, F., Gutierrez, D. M., Nguyen, C. P., Johnson, D. C., Palmer, R. A., Richards, J. B., ... Colston, B. W. (2003). Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology. In T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, & G. E. Cohn (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4958, pp. 22-26) https://doi.org/10.1117/12.485488

Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology. / Chuang, Frank; Gutierrez, Dora M.; Nguyen, Christine P.; Johnson, David C.; Palmer, Richard A.; Richards, James B.; Chang, John T; Visuri, Steven R.; Colston, Bill W.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / T. Vo-Dinh; W.S. Grundfest; D.A. Benaron; G.E. Cohn. Vol. 4958 2003. p. 22-26.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chuang, F, Gutierrez, DM, Nguyen, CP, Johnson, DC, Palmer, RA, Richards, JB, Chang, JT, Visuri, SR & Colston, BW 2003, Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology. in T Vo-Dinh, WS Grundfest, DA Benaron & GE Cohn (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4958, pp. 22-26, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems, San Jose, CA, United States, 1/26/03. https://doi.org/10.1117/12.485488
Chuang F, Gutierrez DM, Nguyen CP, Johnson DC, Palmer RA, Richards JB et al. Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology. In Vo-Dinh T, Grundfest WS, Benaron DA, Cohn GE, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4958. 2003. p. 22-26 https://doi.org/10.1117/12.485488
Chuang, Frank ; Gutierrez, Dora M. ; Nguyen, Christine P. ; Johnson, David C. ; Palmer, Richard A. ; Richards, James B. ; Chang, John T ; Visuri, Steven R. ; Colston, Bill W. / Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology. Proceedings of SPIE - The International Society for Optical Engineering. editor / T. Vo-Dinh ; W.S. Grundfest ; D.A. Benaron ; G.E. Cohn. Vol. 4958 2003. pp. 22-26
@inproceedings{d549a092d40748ab8a2b0ed333689d7b,
title = "Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology",
abstract = "In response to a broad-based need for point-of-care multiplex diagnostic capability, we have developed a novel hybrid platform to analyze optically encoded microspheres arranged on a 2-dimensional planar array. The microspheres which we have initially selected are developed by Luminex Inc. as substrates for sandwich-type fluorescent immunoassays and are typically used in conjunction with a customized flow analyzer. CCD-based optics are the essential feature which enables the development of a rugged diagnostic instrument which can be scaled for point-of-care applications. We have characterized the Multiplex Immunoassay Diagnostic System (MIDS) using a benchtop prototype built around a conventional 12-bit CCD. This system is capable of resolving up to 6 discrete classes of fluorescent microbeads, and measuring their corresponding reporter signal. The MIDS sensitivity to the phycoerythrin (PE) reporter compared favorably to that of the reference Luminex flow system, and is capable of identifying viral, bacterial, and protein simulants in laboratory samples, at concentrations less than 1μg/ml. The ability to resolve small differences in the average PE fluorescence is a direct function of CCD performance, and may be a necessary trade-off for developing a portable and economical detection system. However, we are confident that the MIDS platform can easily be scaled to meet the nominal requirements of any given point-of-care or screening application, and furthermore provide much-needed diagnostic functionality in this particular environment.",
author = "Frank Chuang and Gutierrez, {Dora M.} and Nguyen, {Christine P.} and Johnson, {David C.} and Palmer, {Richard A.} and Richards, {James B.} and Chang, {John T} and Visuri, {Steven R.} and Colston, {Bill W.}",
year = "2003",
doi = "10.1117/12.485488",
language = "English (US)",
volume = "4958",
pages = "22--26",
editor = "T. Vo-Dinh and W.S. Grundfest and D.A. Benaron and G.E. Cohn",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Rapid, point-of-care, multiplex immunodetection using 2-dimensional microarray technology

AU - Chuang, Frank

AU - Gutierrez, Dora M.

AU - Nguyen, Christine P.

AU - Johnson, David C.

AU - Palmer, Richard A.

AU - Richards, James B.

AU - Chang, John T

AU - Visuri, Steven R.

AU - Colston, Bill W.

PY - 2003

Y1 - 2003

N2 - In response to a broad-based need for point-of-care multiplex diagnostic capability, we have developed a novel hybrid platform to analyze optically encoded microspheres arranged on a 2-dimensional planar array. The microspheres which we have initially selected are developed by Luminex Inc. as substrates for sandwich-type fluorescent immunoassays and are typically used in conjunction with a customized flow analyzer. CCD-based optics are the essential feature which enables the development of a rugged diagnostic instrument which can be scaled for point-of-care applications. We have characterized the Multiplex Immunoassay Diagnostic System (MIDS) using a benchtop prototype built around a conventional 12-bit CCD. This system is capable of resolving up to 6 discrete classes of fluorescent microbeads, and measuring their corresponding reporter signal. The MIDS sensitivity to the phycoerythrin (PE) reporter compared favorably to that of the reference Luminex flow system, and is capable of identifying viral, bacterial, and protein simulants in laboratory samples, at concentrations less than 1μg/ml. The ability to resolve small differences in the average PE fluorescence is a direct function of CCD performance, and may be a necessary trade-off for developing a portable and economical detection system. However, we are confident that the MIDS platform can easily be scaled to meet the nominal requirements of any given point-of-care or screening application, and furthermore provide much-needed diagnostic functionality in this particular environment.

AB - In response to a broad-based need for point-of-care multiplex diagnostic capability, we have developed a novel hybrid platform to analyze optically encoded microspheres arranged on a 2-dimensional planar array. The microspheres which we have initially selected are developed by Luminex Inc. as substrates for sandwich-type fluorescent immunoassays and are typically used in conjunction with a customized flow analyzer. CCD-based optics are the essential feature which enables the development of a rugged diagnostic instrument which can be scaled for point-of-care applications. We have characterized the Multiplex Immunoassay Diagnostic System (MIDS) using a benchtop prototype built around a conventional 12-bit CCD. This system is capable of resolving up to 6 discrete classes of fluorescent microbeads, and measuring their corresponding reporter signal. The MIDS sensitivity to the phycoerythrin (PE) reporter compared favorably to that of the reference Luminex flow system, and is capable of identifying viral, bacterial, and protein simulants in laboratory samples, at concentrations less than 1μg/ml. The ability to resolve small differences in the average PE fluorescence is a direct function of CCD performance, and may be a necessary trade-off for developing a portable and economical detection system. However, we are confident that the MIDS platform can easily be scaled to meet the nominal requirements of any given point-of-care or screening application, and furthermore provide much-needed diagnostic functionality in this particular environment.

UR - http://www.scopus.com/inward/record.url?scp=0345714613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345714613&partnerID=8YFLogxK

U2 - 10.1117/12.485488

DO - 10.1117/12.485488

M3 - Conference contribution

AN - SCOPUS:0345714613

VL - 4958

SP - 22

EP - 26

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Vo-Dinh, T.

A2 - Grundfest, W.S.

A2 - Benaron, D.A.

A2 - Cohn, G.E.

ER -