Rapid phenotypic changes in passaged articular chondrocyte subpopulations

Eric M. Darling, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

413 Scopus citations

Abstract

Articular chondrocytes are often expanded in vitro and then used to assist in healing articular cartilage defects. We investigated the extent of dedifferentiation in monolayer-passaged, zonal articular chondrocytes by using quantitative, real-time PCR. The relative gene expressions for collagen type I and II, aggrecan, and superficial zone protein were analyzed for relevant passage numbers (P0-P4) to determine how the expansion of chondrocytes affects the expression of cartilage extracellular matrix proteins. Results reveal that dramatic changes occur as early as first passage. Furthermore, these changes are shown to persist even when the expanded cells are encapsulated in 3D, alginate beads. Successful tissue engineering and autologous cell transplantation procedures rely heavily on having a cell source that expresses the chondrocytic phenotype. The results of this study suggest that major problems exist at the front-end of cartilage regeneration efforts.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalJournal of Orthopaedic Research
Volume23
Issue number2
DOIs
StatePublished - Mar 2005
Externally publishedYes

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Keywords

  • Articular cartilage
  • Chondrocyte
  • Differentiation
  • Gene expression
  • Monolayer expansion
  • Zones

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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