Rapid, non-invasive fluorescence margin assessment: Optical specimen mapping in oral squamous cell carcinoma

Stan van Keulen, Nynke S. van den Berg, Naoki Nishio, Andrew Birkeland, Quan Zhou, Guolan Lu, Han Wei Wang, Lyle Middendorf, Tymour Forouzanfar, Brock A. Martin, A. Dimitrios Colevas, Eben L. Rosenthal

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: Surgical resection remains the primary treatment for the majority of solid tumors. Despite efforts to obtain wide margins, close or positive surgical margins (<5 mm) are found in 15–30% of head and neck cancer patients. Obtaining negative margins requires immediate, intraoperative feedback of margin status. To this end, we propose optical specimen mapping of resected tumor specimens immediately after removal. Materials and methods: A first-in-human pilot study was performed in patients (n = 8) after infusion of fluorescently labeled antibody, panitumumab-IRDye800 to allow surgical mapping of the tumor specimen. Patients underwent standard of care surgical resection for head and neck squamous cell carcinoma (HNSCC). Optical specimen mapping was performed on the primary tumor specimen and correlated with pathological findings after tissue processing. Results: Optical mapping of the specimen had a 95% sensitivity and 89% specificity to detect cancer within 5 mm (n = 160) of the cut surface. To detect tumor within 2 mm of the specimen surface, the sensitivity of optical specimen mapping was 100%. The maximal observed penetration depth of panitumumab-IRDye800 through human tissue in our study was 6.3 mm. Conclusion: Optical specimen mapping is a highly sensitive and specific method for evaluation of margins within <5 mm of the tumor mass in HNSCC specimens. This technology has potentially broad applications for ensuring adequate tumor resection and negative margins in head and neck cancers.

Original languageEnglish (US)
Pages (from-to)58-65
Number of pages8
JournalOral Oncology
Volume88
DOIs
StatePublished - Jan 2019
Externally publishedYes

Fingerprint

Squamous Cell Carcinoma
Fluorescence
Neoplasms
Head and Neck Neoplasms
Standard of Care
Technology
Sensitivity and Specificity
Antibodies

Keywords

  • Fluorescence imaging
  • Molecular imaging
  • Near-infrared
  • Optical specimen mapping
  • Oral cancer
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

Cite this

Rapid, non-invasive fluorescence margin assessment : Optical specimen mapping in oral squamous cell carcinoma. / van Keulen, Stan; van den Berg, Nynke S.; Nishio, Naoki; Birkeland, Andrew; Zhou, Quan; Lu, Guolan; Wang, Han Wei; Middendorf, Lyle; Forouzanfar, Tymour; Martin, Brock A.; Colevas, A. Dimitrios; Rosenthal, Eben L.

In: Oral Oncology, Vol. 88, 01.2019, p. 58-65.

Research output: Contribution to journalArticle

van Keulen, S, van den Berg, NS, Nishio, N, Birkeland, A, Zhou, Q, Lu, G, Wang, HW, Middendorf, L, Forouzanfar, T, Martin, BA, Colevas, AD & Rosenthal, EL 2019, 'Rapid, non-invasive fluorescence margin assessment: Optical specimen mapping in oral squamous cell carcinoma', Oral Oncology, vol. 88, pp. 58-65. https://doi.org/10.1016/j.oraloncology.2018.11.012
van Keulen, Stan ; van den Berg, Nynke S. ; Nishio, Naoki ; Birkeland, Andrew ; Zhou, Quan ; Lu, Guolan ; Wang, Han Wei ; Middendorf, Lyle ; Forouzanfar, Tymour ; Martin, Brock A. ; Colevas, A. Dimitrios ; Rosenthal, Eben L. / Rapid, non-invasive fluorescence margin assessment : Optical specimen mapping in oral squamous cell carcinoma. In: Oral Oncology. 2019 ; Vol. 88. pp. 58-65.
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abstract = "Objective: Surgical resection remains the primary treatment for the majority of solid tumors. Despite efforts to obtain wide margins, close or positive surgical margins (<5 mm) are found in 15–30{\%} of head and neck cancer patients. Obtaining negative margins requires immediate, intraoperative feedback of margin status. To this end, we propose optical specimen mapping of resected tumor specimens immediately after removal. Materials and methods: A first-in-human pilot study was performed in patients (n = 8) after infusion of fluorescently labeled antibody, panitumumab-IRDye800 to allow surgical mapping of the tumor specimen. Patients underwent standard of care surgical resection for head and neck squamous cell carcinoma (HNSCC). Optical specimen mapping was performed on the primary tumor specimen and correlated with pathological findings after tissue processing. Results: Optical mapping of the specimen had a 95{\%} sensitivity and 89{\%} specificity to detect cancer within 5 mm (n = 160) of the cut surface. To detect tumor within 2 mm of the specimen surface, the sensitivity of optical specimen mapping was 100{\%}. The maximal observed penetration depth of panitumumab-IRDye800 through human tissue in our study was 6.3 mm. Conclusion: Optical specimen mapping is a highly sensitive and specific method for evaluation of margins within <5 mm of the tumor mass in HNSCC specimens. This technology has potentially broad applications for ensuring adequate tumor resection and negative margins in head and neck cancers.",
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AU - van Keulen, Stan

AU - van den Berg, Nynke S.

AU - Nishio, Naoki

AU - Birkeland, Andrew

AU - Zhou, Quan

AU - Lu, Guolan

AU - Wang, Han Wei

AU - Middendorf, Lyle

AU - Forouzanfar, Tymour

AU - Martin, Brock A.

AU - Colevas, A. Dimitrios

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AB - Objective: Surgical resection remains the primary treatment for the majority of solid tumors. Despite efforts to obtain wide margins, close or positive surgical margins (<5 mm) are found in 15–30% of head and neck cancer patients. Obtaining negative margins requires immediate, intraoperative feedback of margin status. To this end, we propose optical specimen mapping of resected tumor specimens immediately after removal. Materials and methods: A first-in-human pilot study was performed in patients (n = 8) after infusion of fluorescently labeled antibody, panitumumab-IRDye800 to allow surgical mapping of the tumor specimen. Patients underwent standard of care surgical resection for head and neck squamous cell carcinoma (HNSCC). Optical specimen mapping was performed on the primary tumor specimen and correlated with pathological findings after tissue processing. Results: Optical mapping of the specimen had a 95% sensitivity and 89% specificity to detect cancer within 5 mm (n = 160) of the cut surface. To detect tumor within 2 mm of the specimen surface, the sensitivity of optical specimen mapping was 100%. The maximal observed penetration depth of panitumumab-IRDye800 through human tissue in our study was 6.3 mm. Conclusion: Optical specimen mapping is a highly sensitive and specific method for evaluation of margins within <5 mm of the tumor mass in HNSCC specimens. This technology has potentially broad applications for ensuring adequate tumor resection and negative margins in head and neck cancers.

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KW - Molecular imaging

KW - Near-infrared

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