Abstract
We have developed rapid assays with the potential of being applied to mercury analysis in environmental samples. Our methods combine the simple ELISA-format with the selective, high affinity complexation of mercuric ions by sulfur-containing ligands. The first assay format is based on a sandwich chelate formed by a ligand conjugated to a reporter enzyme, a mercuric ion of the analyzed sample, and a protein bound ligand immobilized on the wells of a microtiter plate. The second assay format involves competition between mercuric ions and an organomercury-conjugate to bind to a chelating conjugate. The assays detect mercuric ions in ppb/high ppt concentrations with high selectivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 38-48 |
| Number of pages | 11 |
| Journal | ACS Symposium Series |
| Volume | 657 |
| State | Published - 1996 |
ASJC Scopus subject areas
- Chemistry(all)