Rapid Mercury Assays

Ferenc Szurdoki, Horacio Kido, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have developed rapid assays with the potential of being applied to mercury analysis in environmental samples. Our methods combine the simple ELISA-format with the selective, high affinity complexation of mercuric ions by sulfur-containing ligands. The first assay format is based on a sandwich chelate formed by a ligand conjugated to a reporter enzyme, a mercuric ion of the analyzed sample, and a protein bound ligand immobilized on the wells of a microtiter plate. The second assay format involves competition between mercuric ions and an organomercury-conjugate to bind to a chelating conjugate. The assays detect mercuric ions in ppb/high ppt concentrations with high selectivity.

Original languageEnglish (US)
Pages (from-to)38-48
Number of pages11
JournalACS Symposium Series
Volume657
StatePublished - 1996

ASJC Scopus subject areas

  • Chemistry(all)

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