Rapid mapping of two genes for resistance to downy mildew from Lactuca serriola to existing clusters of resistance genes

B. Maisonneuve, Y. Bellec, P. Anderson, Richard W Michelmore

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Two resistances to downy mildew derived from Lactuca serriola were characterized genetically and mapped using molecular markers. Classical genetic analysis suggested monogenic inheritance; however, the presence of multiple, tightly-linked genes in each case could not be eliminated. Therefore, they were designated resistance factors R17 and R18. Analysis with molecular markers known to be linked to clusters of resistance genes quickly revealed linkage of R18 to the major cluster of resistance genes and provided six linked markers, three RAPD (Random Amplified Polymorphic DNA) markers and three codominant SCAR (Sequence Characterized Amplified Region) markers. The mapping of R17 required the screening of arbitrary RAPD markers using bulked segregant analysis; this provided five linked markers, three of which segregated in the basic mapping population. This demonstrated loose linkage to a second cluster of resistance genes and provided additional linked markers. Two RAPD markers linked to R17 were converted into SCARs. The identification of reliable PCR-based markers flanking each gene will aid in selection and in combining these resistance genes with others.

Original languageEnglish (US)
Pages (from-to)96-104
Number of pages9
JournalTheoretical And Applied Genetics
Volume89
Issue number1
DOIs
StatePublished - Sep 1994

Fingerprint

Lactuca serriola
Chromosome Mapping
downy mildew
Multigene Family
Genetic Markers
random amplified polymorphic DNA technique
genetic markers
Genes
genes
R Factors
linkage (genetics)
monogenic inheritance
Polymerase Chain Reaction
Population
genetic techniques and protocols
screening

Keywords

  • Downy mildew Lactuca serriola
  • Lettuce
  • RAPDs
  • SCAR

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Rapid mapping of two genes for resistance to downy mildew from Lactuca serriola to existing clusters of resistance genes. / Maisonneuve, B.; Bellec, Y.; Anderson, P.; Michelmore, Richard W.

In: Theoretical And Applied Genetics, Vol. 89, No. 1, 09.1994, p. 96-104.

Research output: Contribution to journalArticle

Maisonneuve, B. ; Bellec, Y. ; Anderson, P. ; Michelmore, Richard W. / Rapid mapping of two genes for resistance to downy mildew from Lactuca serriola to existing clusters of resistance genes. In: Theoretical And Applied Genetics. 1994 ; Vol. 89, No. 1. pp. 96-104.
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