Abstract
High-performance liquid chromatography with fluorescence detection has been utilized for the rapid determination of total homocysteine, cysteine, and cysteinylglycine in human serum and plasma. Our earlier procedure (Anal Biochem 1989; 178:208), which used monobromobimane to specifically derivatize thiols, has been extensively modified to allow for rapid processing of samples. As a result, >80 samples a day can be assayed for total homocysteine, cysteine, and cysteinylglycine. The method is sensitive (lower limit of detection ≤4 pmol in the assay) and precise (intra- and interassay CV for homocysteine, 3.31% and 4.85%, respectively). Mean total homocysteine concentrations in plasma and serum were significantly different, both from healthy male donors (9.26 and 12.30 μmol/L, respectively; P <0.001) and healthy female donors (7.85 and 10.34 μmol/L, respectively; P <0.001). The differences in total homocysteine between sexes were also significant (P = 0.002 for both plasma and serum). Similar differences were found for cysteine and cysteinylglycine. We found a significant inverse correlation between serum cobalamin and total homocysteine in men (P = 0.0102) and women (P = 0.0174). Serum folate also inversely correlated with total homocysteine in both sexes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 873-881 |
| Number of pages | 9 |
| Journal | Clinical Chemistry |
| Volume | 40 |
| Issue number | 6 |
| State | Published - 1994 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Rapid HPLC determination of total homocysteine and other thiols in serum and plasma : Sex differences and correlation with cobalamin and folate concentrations in healthy subjects. / Jacobsen, Donald W.; Gatautis, Vytenis J.; Green, Ralph; Robinson, Killian; Savon, Susan R.; Secic, Michelle; Ji, Ji; Otto, Joanne M.; Taylor, Lloyd M.
In: Clinical Chemistry, Vol. 40, No. 6, 1994, p. 873-881.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid HPLC determination of total homocysteine and other thiols in serum and plasma
T2 - Sex differences and correlation with cobalamin and folate concentrations in healthy subjects
AU - Jacobsen, Donald W.
AU - Gatautis, Vytenis J.
AU - Green, Ralph
AU - Robinson, Killian
AU - Savon, Susan R.
AU - Secic, Michelle
AU - Ji, Ji
AU - Otto, Joanne M.
AU - Taylor, Lloyd M.
PY - 1994
Y1 - 1994
N2 - High-performance liquid chromatography with fluorescence detection has been utilized for the rapid determination of total homocysteine, cysteine, and cysteinylglycine in human serum and plasma. Our earlier procedure (Anal Biochem 1989; 178:208), which used monobromobimane to specifically derivatize thiols, has been extensively modified to allow for rapid processing of samples. As a result, >80 samples a day can be assayed for total homocysteine, cysteine, and cysteinylglycine. The method is sensitive (lower limit of detection ≤4 pmol in the assay) and precise (intra- and interassay CV for homocysteine, 3.31% and 4.85%, respectively). Mean total homocysteine concentrations in plasma and serum were significantly different, both from healthy male donors (9.26 and 12.30 μmol/L, respectively; P <0.001) and healthy female donors (7.85 and 10.34 μmol/L, respectively; P <0.001). The differences in total homocysteine between sexes were also significant (P = 0.002 for both plasma and serum). Similar differences were found for cysteine and cysteinylglycine. We found a significant inverse correlation between serum cobalamin and total homocysteine in men (P = 0.0102) and women (P = 0.0174). Serum folate also inversely correlated with total homocysteine in both sexes.
AB - High-performance liquid chromatography with fluorescence detection has been utilized for the rapid determination of total homocysteine, cysteine, and cysteinylglycine in human serum and plasma. Our earlier procedure (Anal Biochem 1989; 178:208), which used monobromobimane to specifically derivatize thiols, has been extensively modified to allow for rapid processing of samples. As a result, >80 samples a day can be assayed for total homocysteine, cysteine, and cysteinylglycine. The method is sensitive (lower limit of detection ≤4 pmol in the assay) and precise (intra- and interassay CV for homocysteine, 3.31% and 4.85%, respectively). Mean total homocysteine concentrations in plasma and serum were significantly different, both from healthy male donors (9.26 and 12.30 μmol/L, respectively; P <0.001) and healthy female donors (7.85 and 10.34 μmol/L, respectively; P <0.001). The differences in total homocysteine between sexes were also significant (P = 0.002 for both plasma and serum). Similar differences were found for cysteine and cysteinylglycine. We found a significant inverse correlation between serum cobalamin and total homocysteine in men (P = 0.0102) and women (P = 0.0174). Serum folate also inversely correlated with total homocysteine in both sexes.
UR - http://www.scopus.com/inward/record.url?scp=0028284362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028284362&partnerID=8YFLogxK
M3 - Article
C2 - 8087981
AN - SCOPUS:0028284362
VL - 40
SP - 873
EP - 881
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 6
ER -