Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

Farzad Fereidouni, Dimitris Gorpas, Dinglong Ma, Hussain Fatakdawala, Laura Marcu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time-the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.

Original languageEnglish (US)
Number of pages1
JournalMethods and Applications in Fluorescence
Volume5
Issue number3
DOIs
StatePublished - Sep 1 2017

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Materials Science(all)
  • Spectroscopy

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