Rapid expression of fibronectin in the rabbit heart after myocardial infarction with and without reperfusion

Anne A Knowlton, Carolyn M. Connelly, Gabriel M. Romo, Wilfred Mamuya, Carl S. Apstein, Peter Brecher, Soeun Ngoy

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

The expression of flbronectin in the repair process after myocardial infarction was studied using two protocols of coronary occlusion in the rabbit: a permanent occlusion or 3 h of occlusion followed by reperfusion (too late for salvage). We found a rapid and progressive increase in cardiac fibronectin expression in the infarcted region of the ventricle. Steady-state mRNA levels for flbronectin increased 13- and 16-fold, respectively, in the permanent and reperfused infarcts 1 d postinfarction. Immunological detection of the protein with a polyclonal antibody against plasma flbronectin showed significant increases of the protein flbronectin in the infarcted myocardium by day 3 in the reperfused group and by day 5 in the permanent coronary occlusion group. Ribonuclease protection assays established the induction of EIIIB containing flbronectin mRNA in both models by day 1 and use of a monoclonal antibody showed an increase in the EIIIA isoform 2 d postinfarction. Increases in steady-state mRNA levels for several collagen types were found in both groups, but these changes occurred after those noted for flbronectin. Thus fibronectin mRNA and protein expression increased rapidly postinfarction suggesting a functional role in the repair process.

Original languageEnglish (US)
Pages (from-to)1060-1068
Number of pages9
JournalJournal of Clinical Investigation
Volume89
Issue number4
StatePublished - 1992
Externally publishedYes

Keywords

  • Collagen
  • Extracellular matrix
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Infarct healing
  • Wound healing

ASJC Scopus subject areas

  • Medicine(all)

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    Knowlton, A. A., Connelly, C. M., Romo, G. M., Mamuya, W., Apstein, C. S., Brecher, P., & Ngoy, S. (1992). Rapid expression of fibronectin in the rabbit heart after myocardial infarction with and without reperfusion. Journal of Clinical Investigation, 89(4), 1060-1068.