Rapid enzyme immunoassay for measurement of bovine progesterone

Rodney W. Claycomb, Michael J. Delwiche, Coralie J. Munro, Robert Bondurant

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.

Original languageEnglish (US)
Pages (from-to)1165-1171
Number of pages7
JournalBiosensors and Bioelectronics
Volume13
Issue number11
DOIs
StatePublished - Nov 1 1998

Keywords

  • Antibody
  • Cow
  • Estrus
  • Hormone

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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