Rapid enzyme immunoassay for measurement of bovine progesterone

Rodney W. Claycomb; Michael J. Delwiche; Coralie J. Munro; Robert Bondurant

doi:10.1016/S0956-5663(98)00081-5

Rapid enzyme immunoassay for measurement of bovine progesterone

Rodney W. Claycomb, Michael J. Delwiche, Coralie J. Munro, Robert Bondurant

School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.

Original language	English (US)
Pages (from-to)	1165-1171
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	13
Issue number	11
DOIs	https://doi.org/10.1016/S0956-5663(98)00081-5
State	Published - Nov 1 1998

Keywords

Antibody
Cow
Estrus
Hormone

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Electrochemistry

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title = "Rapid enzyme immunoassay for measurement of bovine progesterone",

abstract = "Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.",

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AU - Claycomb, Rodney W.

AU - Delwiche, Michael J.

AU - Munro, Coralie J.

AU - Bondurant, Robert

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AB - Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.

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KW - Estrus

KW - Hormone

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