Abstract
Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.
Original language | English (US) |
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Pages (from-to) | 1165-1171 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 13 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 1998 |
Keywords
- Antibody
- Cow
- Estrus
- Hormone
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Electrochemistry