Abstract
A rapid, selective, and sensitive LC-MS/MS method was developed for the quantitative determination of domoic acid in serum and urine samples. Samples were prepared for analysis using an Oasis HLB SPE column. Determination was by a reversed phase HPLC using a mixture of methanol, acetonitrile, and water containing 1% acetic acid and an electrospray ionization (ESI) ion-trap mass spectrometer (Finnigan LCQ). The method was validated by analyzing five replicates each of negative control bovine serum or urine fortified with domoic acid at the 0.005 μg/g method detection limit (MDL) and at the 0.05 μg/g level. Recoveries ranged from 90 to 95% for fortifications at the MDL and from 92 to 98% for fortifications 10 times higher than the MDL. The diagnostic utility of the method was tested by analyzing samples from live animals showing clinical signs suggestive of domoic acid poisoning submitted to the veterinary toxicology laboratory.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1791-1796 |
| Number of pages | 6 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 51 |
| Issue number | 7 |
| DOIs | |
| State | Published - Mar 26 2003 |
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Keywords
- Domoic acid
- HPLC
- LC/MS
- Mass spectrometry
- Serum
- Urine
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Rapid determination of domoic acid in serum and urine by liquid chromatography-electrospray tandem mass spectrometry. / Tor, Elizabeth R.; Puschner, Birgit; Whitehead, William E.
In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 7, 26.03.2003, p. 1791-1796.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid determination of domoic acid in serum and urine by liquid chromatography-electrospray tandem mass spectrometry
AU - Tor, Elizabeth R.
AU - Puschner, Birgit
AU - Whitehead, William E.
PY - 2003/3/26
Y1 - 2003/3/26
N2 - A rapid, selective, and sensitive LC-MS/MS method was developed for the quantitative determination of domoic acid in serum and urine samples. Samples were prepared for analysis using an Oasis HLB SPE column. Determination was by a reversed phase HPLC using a mixture of methanol, acetonitrile, and water containing 1% acetic acid and an electrospray ionization (ESI) ion-trap mass spectrometer (Finnigan LCQ). The method was validated by analyzing five replicates each of negative control bovine serum or urine fortified with domoic acid at the 0.005 μg/g method detection limit (MDL) and at the 0.05 μg/g level. Recoveries ranged from 90 to 95% for fortifications at the MDL and from 92 to 98% for fortifications 10 times higher than the MDL. The diagnostic utility of the method was tested by analyzing samples from live animals showing clinical signs suggestive of domoic acid poisoning submitted to the veterinary toxicology laboratory.
AB - A rapid, selective, and sensitive LC-MS/MS method was developed for the quantitative determination of domoic acid in serum and urine samples. Samples were prepared for analysis using an Oasis HLB SPE column. Determination was by a reversed phase HPLC using a mixture of methanol, acetonitrile, and water containing 1% acetic acid and an electrospray ionization (ESI) ion-trap mass spectrometer (Finnigan LCQ). The method was validated by analyzing five replicates each of negative control bovine serum or urine fortified with domoic acid at the 0.005 μg/g method detection limit (MDL) and at the 0.05 μg/g level. Recoveries ranged from 90 to 95% for fortifications at the MDL and from 92 to 98% for fortifications 10 times higher than the MDL. The diagnostic utility of the method was tested by analyzing samples from live animals showing clinical signs suggestive of domoic acid poisoning submitted to the veterinary toxicology laboratory.
KW - Domoic acid
KW - HPLC
KW - LC/MS
KW - Mass spectrometry
KW - Serum
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=0037467455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037467455&partnerID=8YFLogxK
U2 - 10.1021/jf020947f
DO - 10.1021/jf020947f
M3 - Article
C2 - 12643631
AN - SCOPUS:0037467455
VL - 51
SP - 1791
EP - 1796
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 7
ER -