Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis

Stephen M Griffey, Susan A. Kraegel, Bruce R. Madewell

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A total of 126 spontaneous lung tumors from pet dogs were examined for K-ras mutations within exon 1 and exon 2 using a non-radioisotope single-strand conformational polymorphism analysis (SSCP) detection method on PCR products. Mutations were confirmed by direct DNA sequencing. Tumors were classified as adenomas (9), bronchioloalveolar carcinomas (59), adenocarcinomas (30), adenosquamous carcinomas (16), squamous cell carcinomas (3) and anaplastic carcinomas (9). Nineteen mutations were detected in the malignant tumors: 18 occurred in exon 1 codon 12 and one in exon 2 codon 61. No mutations were present in the adenomas. The most common mutation was a G→A transition (11/19) in the second position of codon 12. Based on this study, K-ras mutations occur in canine non-small cell lung carcinomas. The frequency and type of mutation more closely matches tumors from human non-smokers with K-ras mutations than smokers. With the application of screening techniques such as SSCP, large numbers of dog tumors can be examined to provide a large animal model for comparative studies of carcinogenesis.

Original languageEnglish (US)
Pages (from-to)959-963
Number of pages5
JournalCarcinogenesis
Volume19
Issue number6
DOIs
StatePublished - Jun 1998

Fingerprint

ras Genes
Canidae
Lung Neoplasms
Mutation
Exons
Codon
Neoplasms
Adenoma
Dogs
Adenosquamous Carcinoma
Bronchiolo-Alveolar Adenocarcinoma
Pets
Mutation Rate
DNA Sequence Analysis
Non-Small Cell Lung Carcinoma
Squamous Cell Carcinoma
Carcinogenesis
Adenocarcinoma
Animal Models
Carcinoma

ASJC Scopus subject areas

  • Cancer Research

Cite this

Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis. / Griffey, Stephen M; Kraegel, Susan A.; Madewell, Bruce R.

In: Carcinogenesis, Vol. 19, No. 6, 06.1998, p. 959-963.

Research output: Contribution to journalArticle

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