Rapid decay of serum IgG recognizing gram-negative cell wall core antigens in neonatal calves.

V. L. Douglas, James S Cullor, J. W. Tyler, Mark Thurmond, R. B. Bushnell

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Serum immunoglobulins of the IgG isotype recognizing common gram-negative cell core epitopes were serially measured, using a direct ELISA, on samples obtained from 20 neonatal Holstein calves. An R-mutant Escherichia coli (strain J5) was used as a plate antigen in this assay. Total serum IgG concentration was measured using radial immunodiffusion. Half-lives of core antigen-specific IgG (7.56 days) and total serum IgG (22.66 days) were dramatically different (P less than 0.0005). This may be an indication of cross-reactive consumption of core antigen-specific immunoglobulins.

Original languageEnglish (US)
Pages (from-to)1138-1140
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume50
Issue number7
StatePublished - Jul 1989

Fingerprint

Cell Wall
Immunoglobulin G
deterioration
cell walls
calves
antigens
Antigens
immunoglobulins
Serum
epitopes
half life
Immunoglobulin Isotypes
Immunodiffusion
Holstein
enzyme-linked immunosorbent assay
Escherichia coli
Immunoglobulins
Epitopes
mutants
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Rapid decay of serum IgG recognizing gram-negative cell wall core antigens in neonatal calves. / Douglas, V. L.; Cullor, James S; Tyler, J. W.; Thurmond, Mark; Bushnell, R. B.

In: American Journal of Veterinary Research, Vol. 50, No. 7, 07.1989, p. 1138-1140.

Research output: Contribution to journalArticle

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