Rapid and specific detection of Asian- and African-lineage Zika viruses

Nunya Chotiwan, Connie D. Brewster, Tereza Magalhaes, James Weger-Lucarelli, Nisha K. Duggal, Claudia Rückert, Chilinh Nguyen, Selene M.Garcia Luna, Joseph R. Fauver, Barb Andre, Meg Gray, William C.Black Iv, Rebekah C. Kading, Gregory D. Ebel, Guillermina Kuan, Angel Balmaseda, Thomas Jaenisch, Ernesto T.A. Marques, Aaron Brault, Eva HarrisBrian D. Foy, Sandra L. Quackenbush, Rushika Perera, Joel Rovnak

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017

Original languageEnglish (US)
Article numberaag0538
JournalScience Translational Medicine
Volume9
Issue number388
DOIs
StatePublished - May 3 2017
Externally publishedYes

Fingerprint

Semen
Chikungunya virus
RNA
Yellow Fever
Flavivirus
West Nile virus
Dengue
Culicidae
Saliva
Reverse Transcription
Cultured Cells
Zika Virus
Urine
Viruses
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chotiwan, N., Brewster, C. D., Magalhaes, T., Weger-Lucarelli, J., Duggal, N. K., Rückert, C., ... Rovnak, J. (2017). Rapid and specific detection of Asian- and African-lineage Zika viruses. Science Translational Medicine, 9(388), [aag0538]. https://doi.org/10.1126/scitranslmed.aag0538

Rapid and specific detection of Asian- and African-lineage Zika viruses. / Chotiwan, Nunya; Brewster, Connie D.; Magalhaes, Tereza; Weger-Lucarelli, James; Duggal, Nisha K.; Rückert, Claudia; Nguyen, Chilinh; Luna, Selene M.Garcia; Fauver, Joseph R.; Andre, Barb; Gray, Meg; Iv, William C.Black; Kading, Rebekah C.; Ebel, Gregory D.; Kuan, Guillermina; Balmaseda, Angel; Jaenisch, Thomas; Marques, Ernesto T.A.; Brault, Aaron; Harris, Eva; Foy, Brian D.; Quackenbush, Sandra L.; Perera, Rushika; Rovnak, Joel.

In: Science Translational Medicine, Vol. 9, No. 388, aag0538, 03.05.2017.

Research output: Contribution to journalArticle

Chotiwan, N, Brewster, CD, Magalhaes, T, Weger-Lucarelli, J, Duggal, NK, Rückert, C, Nguyen, C, Luna, SMG, Fauver, JR, Andre, B, Gray, M, Iv, WCB, Kading, RC, Ebel, GD, Kuan, G, Balmaseda, A, Jaenisch, T, Marques, ETA, Brault, A, Harris, E, Foy, BD, Quackenbush, SL, Perera, R & Rovnak, J 2017, 'Rapid and specific detection of Asian- and African-lineage Zika viruses', Science Translational Medicine, vol. 9, no. 388, aag0538. https://doi.org/10.1126/scitranslmed.aag0538
Chotiwan N, Brewster CD, Magalhaes T, Weger-Lucarelli J, Duggal NK, Rückert C et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Science Translational Medicine. 2017 May 3;9(388). aag0538. https://doi.org/10.1126/scitranslmed.aag0538
Chotiwan, Nunya ; Brewster, Connie D. ; Magalhaes, Tereza ; Weger-Lucarelli, James ; Duggal, Nisha K. ; Rückert, Claudia ; Nguyen, Chilinh ; Luna, Selene M.Garcia ; Fauver, Joseph R. ; Andre, Barb ; Gray, Meg ; Iv, William C.Black ; Kading, Rebekah C. ; Ebel, Gregory D. ; Kuan, Guillermina ; Balmaseda, Angel ; Jaenisch, Thomas ; Marques, Ernesto T.A. ; Brault, Aaron ; Harris, Eva ; Foy, Brian D. ; Quackenbush, Sandra L. ; Perera, Rushika ; Rovnak, Joel. / Rapid and specific detection of Asian- and African-lineage Zika viruses. In: Science Translational Medicine. 2017 ; Vol. 9, No. 388.
@article{692a950e34ea47c3bf8d1c22531026d1,
title = "Rapid and specific detection of Asian- and African-lineage Zika viruses",
abstract = "Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017",
author = "Nunya Chotiwan and Brewster, {Connie D.} and Tereza Magalhaes and James Weger-Lucarelli and Duggal, {Nisha K.} and Claudia R{\"u}ckert and Chilinh Nguyen and Luna, {Selene M.Garcia} and Fauver, {Joseph R.} and Barb Andre and Meg Gray and Iv, {William C.Black} and Kading, {Rebekah C.} and Ebel, {Gregory D.} and Guillermina Kuan and Angel Balmaseda and Thomas Jaenisch and Marques, {Ernesto T.A.} and Aaron Brault and Eva Harris and Foy, {Brian D.} and Quackenbush, {Sandra L.} and Rushika Perera and Joel Rovnak",
year = "2017",
month = "5",
day = "3",
doi = "10.1126/scitranslmed.aag0538",
language = "English (US)",
volume = "9",
journal = "Science Translational Medicine",
issn = "1946-6234",
publisher = "American Association for the Advancement of Science",
number = "388",

}

TY - JOUR

T1 - Rapid and specific detection of Asian- and African-lineage Zika viruses

AU - Chotiwan, Nunya

AU - Brewster, Connie D.

AU - Magalhaes, Tereza

AU - Weger-Lucarelli, James

AU - Duggal, Nisha K.

AU - Rückert, Claudia

AU - Nguyen, Chilinh

AU - Luna, Selene M.Garcia

AU - Fauver, Joseph R.

AU - Andre, Barb

AU - Gray, Meg

AU - Iv, William C.Black

AU - Kading, Rebekah C.

AU - Ebel, Gregory D.

AU - Kuan, Guillermina

AU - Balmaseda, Angel

AU - Jaenisch, Thomas

AU - Marques, Ernesto T.A.

AU - Brault, Aaron

AU - Harris, Eva

AU - Foy, Brian D.

AU - Quackenbush, Sandra L.

AU - Perera, Rushika

AU - Rovnak, Joel

PY - 2017/5/3

Y1 - 2017/5/3

N2 - Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017

AB - Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017

UR - http://www.scopus.com/inward/record.url?scp=85019265809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019265809&partnerID=8YFLogxK

U2 - 10.1126/scitranslmed.aag0538

DO - 10.1126/scitranslmed.aag0538

M3 - Article

C2 - 28469032

AN - SCOPUS:85019265809

VL - 9

JO - Science Translational Medicine

JF - Science Translational Medicine

SN - 1946-6234

IS - 388

M1 - aag0538

ER -