Abstract
Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017
| Original language | English (US) |
|---|---|
| Article number | aag0538 |
| Journal | Science Translational Medicine |
| Volume | 9 |
| Issue number | 388 |
| DOIs | |
| State | Published - May 3 2017 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Rapid and specific detection of Asian- and African-lineage Zika viruses. / Chotiwan, Nunya; Brewster, Connie D.; Magalhaes, Tereza; Weger-Lucarelli, James; Duggal, Nisha K.; Rückert, Claudia; Nguyen, Chilinh; Luna, Selene M.Garcia; Fauver, Joseph R.; Andre, Barb; Gray, Meg; Iv, William C.Black; Kading, Rebekah C.; Ebel, Gregory D.; Kuan, Guillermina; Balmaseda, Angel; Jaenisch, Thomas; Marques, Ernesto T.A.; Brault, Aaron; Harris, Eva; Foy, Brian D.; Quackenbush, Sandra L.; Perera, Rushika; Rovnak, Joel.
In: Science Translational Medicine, Vol. 9, No. 388, aag0538, 03.05.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid and specific detection of Asian- and African-lineage Zika viruses
AU - Chotiwan, Nunya
AU - Brewster, Connie D.
AU - Magalhaes, Tereza
AU - Weger-Lucarelli, James
AU - Duggal, Nisha K.
AU - Rückert, Claudia
AU - Nguyen, Chilinh
AU - Luna, Selene M.Garcia
AU - Fauver, Joseph R.
AU - Andre, Barb
AU - Gray, Meg
AU - Iv, William C.Black
AU - Kading, Rebekah C.
AU - Ebel, Gregory D.
AU - Kuan, Guillermina
AU - Balmaseda, Angel
AU - Jaenisch, Thomas
AU - Marques, Ernesto T.A.
AU - Brault, Aaron
AU - Harris, Eva
AU - Foy, Brian D.
AU - Quackenbush, Sandra L.
AU - Perera, Rushika
AU - Rovnak, Joel
PY - 2017/5/3
Y1 - 2017/5/3
N2 - Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017
AB - Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. 2017
UR - http://www.scopus.com/inward/record.url?scp=85019265809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019265809&partnerID=8YFLogxK
U2 - 10.1126/scitranslmed.aag0538
DO - 10.1126/scitranslmed.aag0538
M3 - Article
C2 - 28469032
AN - SCOPUS:85019265809
VL - 9
JO - Science Translational Medicine
JF - Science Translational Medicine
SN - 1946-6234
IS - 388
M1 - aag0538
ER -