Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis

Rance B Lefebvre, J. W. Foley, A. B. Thiermann

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 μg of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Chromosomal DNA from dinger zones (visible subsurface zones of leptospiral growth) of first semisolid subcultures of field isolates was also isolated and characterized, thus greatly speeding up the diagnostic process.

Original languageEnglish (US)
Pages (from-to)606-608
Number of pages3
JournalJournal of Clinical Microbiology
Volume22
Issue number4
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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