Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157: H7 in raw milk and ice-cream

Christopher M. Gooding, Prabhakara V Choudary

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalJournal of Dairy Research
Volume64
Issue number1
DOIs
StatePublished - Feb 1997

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Applied Microbiology and Biotechnology
  • Food Science

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