TY - JOUR
T1 - Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157
T2 - H7 in raw milk and ice-cream
AU - Gooding, Christopher M.
AU - Choudary, Prabhakara V
PY - 1997/2
Y1 - 1997/2
N2 - Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.
AB - Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.
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U2 - 10.1017/S0022029996001896
DO - 10.1017/S0022029996001896
M3 - Article
C2 - 9120079
AN - SCOPUS:0031062370
VL - 64
SP - 87
EP - 93
JO - Journal of Dairy Research
JF - Journal of Dairy Research
SN - 0022-0299
IS - 1
ER -