Abstract
Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 87-93 |
| Number of pages | 7 |
| Journal | Journal of Dairy Research |
| Volume | 64 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1997 |
ASJC Scopus subject areas
- Animal Science and Zoology
- Applied Microbiology and Biotechnology
- Food Science
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Gooding, C. M., & Choudary, P. V. (1997). Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157: H7 in raw milk and ice-cream. Journal of Dairy Research, 64(1), 87-93. https://doi.org/10.1017/S0022029996001896