Rapid and sensitive enzyme-linked immunosorbent assay for the microsomal epoxide hydrolase

Sarjeet S. Gill, Siong I. Wie, Thomas M. Guenthner, Franz Oesch, Bruce D. Hammock

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A rapid and sensitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for micrsomal epoxide hydrolase of rat liver. The assay, which is easily and readily performed, is significantly more sensitive than most enzymatic epoxide hydrolase assays routinely used and electroimmunoassays previously developed. The limit of sensitivity of the ELISA is between 2-5 ng of microsomal epoxide hydrolase. Using the ELISA microsomal epoxide hydrolases of mouse and rat liver were shown to be antigenically very similar, while microsomal epoxide hydrolases of Guinea pig, monkey and human liver are antigenically distinct from those of rat and mouse. The ELISA developed here is capable of detecting microsomal epoxide hydrolase of rat and mouse liver even when significant enzymatic activity is lost. These results indicate that the antigenic sites recognized by the antibodies used are distinct from the catalytic site of the epoxide hydrolase. Approximately 1.9% of rat microsomal protein was quantified as microsomal epoxide hydrolase by the ELISA. Low levels of microsomal epoxide hydrolase were also detected in rat liver cytosol (∼0.02% of the cytosolic protein) demonstrating that microsomal epoxide hydrolase is not totally membrane bound or that an immunologically related protein occurs in the cytosol of normal rat liver. The ELISA developed here will be valuable in investigating further the role of microsomal epoxide hydrolase.

Original languageEnglish (US)
Pages (from-to)1307-1310
Number of pages4
JournalCarcinogenesis
Volume3
Issue number11
DOIs
StatePublished - 1982

Fingerprint

Epoxide Hydrolases
Enzyme-linked Immunosorbent Assay
Hydrolases
Assays
Liver
Enzymes
Enzyme-Linked Immunosorbent Assay
Rats
mouse EPHX1 protein
Mouse
Protein
Cytosol
Distinct
Proteins
Guinea pig
Immunosorbents
Enzyme-linked immunosorbent assay
Rat
Antibody
Membrane

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Gill, S. S., Wie, S. I., Guenthner, T. M., Oesch, F., & Hammock, B. D. (1982). Rapid and sensitive enzyme-linked immunosorbent assay for the microsomal epoxide hydrolase. Carcinogenesis, 3(11), 1307-1310. https://doi.org/10.1093/carcin/3.11.1307

Rapid and sensitive enzyme-linked immunosorbent assay for the microsomal epoxide hydrolase. / Gill, Sarjeet S.; Wie, Siong I.; Guenthner, Thomas M.; Oesch, Franz; Hammock, Bruce D.

In: Carcinogenesis, Vol. 3, No. 11, 1982, p. 1307-1310.

Research output: Contribution to journalArticle

Gill, SS, Wie, SI, Guenthner, TM, Oesch, F & Hammock, BD 1982, 'Rapid and sensitive enzyme-linked immunosorbent assay for the microsomal epoxide hydrolase', Carcinogenesis, vol. 3, no. 11, pp. 1307-1310. https://doi.org/10.1093/carcin/3.11.1307
Gill, Sarjeet S. ; Wie, Siong I. ; Guenthner, Thomas M. ; Oesch, Franz ; Hammock, Bruce D. / Rapid and sensitive enzyme-linked immunosorbent assay for the microsomal epoxide hydrolase. In: Carcinogenesis. 1982 ; Vol. 3, No. 11. pp. 1307-1310.
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