Rapid and quantitative DNA analysis of genetic mutations for polycystic kidney disease (PKD) using magnetic/luminescent nanoparticles

Ahjeong Son, Amy Dhirapong, Dosi K. Dosev, Ian M. Kennedy, Robert H Weiss, Krassimira R. Hristova

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Rapid and accurate detection of genetic mutations based on nanotechnology would provide substantial advances in detection of polycystic kidney disease (PKD), a disease whose current methods of detection are cumbersome due to the large size and duplication of the mutated gene. In this study, a nanotechnology-based DNA assay was developed for detection of SNPs (single nucleotide polymorphisms) in a feline autosomal dominant PKD (ADPKD) model which can readily be adapted to diagnosis of human ADPKD type 1. Europium and terbium phosphors were doped into gadolinium crystal hosts with a magnetic core, providing stable luminescence and the possibility of magnetic manipulations in a solution-based assay. A hybridization-in-solution DNA assay was optimized for feline PKD gene SNP detection using genomic DNA extracted from feline kidney tissue and blood. This assay showed a substantial differentiation between PKD and control specimens. The nanotechnology-based DNA assay is attractive from the viewpoint of rapid availability, simple methodology, and cost reduction for clinical use to detect mutations involved in human ADPKD and other genetic diseases. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1829-1835
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume390
Issue number7
DOIs
StatePublished - Apr 2008

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Keywords

  • DNA
  • Hybridization-in-solution
  • Lanthanide oxide
  • Nanoparticles
  • Polycystic kidney disease (PKD)
  • Single nucleotide polymorphisms (SNPs)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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