Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1

K. W. Hunter, S. D. Ontiveros, M. L. Watson, V. P. Stanton, P. Gutierrez, D. Bhat, J. Rochelle, S. Graw, M. Schalling, H. Aburatani, S. D M Brown, Michael F Seldin, D. E. Housman

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.

Original languageEnglish (US)
Pages (from-to)597-607
Number of pages11
JournalMammalian Genome
Volume5
Issue number10
DOIs
StatePublished - Oct 1994
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Hunter, K. W., Ontiveros, S. D., Watson, M. L., Stanton, V. P., Gutierrez, P., Bhat, D., Rochelle, J., Graw, S., Schalling, M., Aburatani, H., Brown, S. D. M., Seldin, M. F., & Housman, D. E. (1994). Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1. Mammalian Genome, 5(10), 597-607. https://doi.org/10.1007/BF00411453