Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1

K. W. Hunter, S. D. Ontiveros, M. L. Watson, V. P. Stanton, P. Gutierrez, D. Bhat, J. Rochelle, S. Graw, M. Schalling, H. Aburatani, S. D M Brown, Michael F Seldin, D. E. Housman

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.

Original languageEnglish (US)
Pages (from-to)597-607
Number of pages11
JournalMammalian Genome
Volume5
Issue number10
DOIs
StatePublished - Oct 1994
Externally publishedYes

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Yeast Artificial Chromosomes
Interspersed Repetitive Sequences
Chromosomes, Human, Pair 1
Clone Cells
Chromosomes
Genome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR) : Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1. / Hunter, K. W.; Ontiveros, S. D.; Watson, M. L.; Stanton, V. P.; Gutierrez, P.; Bhat, D.; Rochelle, J.; Graw, S.; Schalling, M.; Aburatani, H.; Brown, S. D M; Seldin, Michael F; Housman, D. E.

In: Mammalian Genome, Vol. 5, No. 10, 10.1994, p. 597-607.

Research output: Contribution to journalArticle

Hunter, KW, Ontiveros, SD, Watson, ML, Stanton, VP, Gutierrez, P, Bhat, D, Rochelle, J, Graw, S, Schalling, M, Aburatani, H, Brown, SDM, Seldin, MF & Housman, DE 1994, 'Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1', Mammalian Genome, vol. 5, no. 10, pp. 597-607. https://doi.org/10.1007/BF00411453
Hunter, K. W. ; Ontiveros, S. D. ; Watson, M. L. ; Stanton, V. P. ; Gutierrez, P. ; Bhat, D. ; Rochelle, J. ; Graw, S. ; Schalling, M. ; Aburatani, H. ; Brown, S. D M ; Seldin, Michael F ; Housman, D. E. / Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR) : Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1. In: Mammalian Genome. 1994 ; Vol. 5, No. 10. pp. 597-607.
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