Abstract
We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 597-607 |
| Number of pages | 11 |
| Journal | Mammalian Genome |
| Volume | 5 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR) : Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1. / Hunter, K. W.; Ontiveros, S. D.; Watson, M. L.; Stanton, V. P.; Gutierrez, P.; Bhat, D.; Rochelle, J.; Graw, S.; Schalling, M.; Aburatani, H.; Brown, S. D M; Seldin, Michael F; Housman, D. E.
In: Mammalian Genome, Vol. 5, No. 10, 10.1994, p. 597-607.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR)
T2 - Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1
AU - Hunter, K. W.
AU - Ontiveros, S. D.
AU - Watson, M. L.
AU - Stanton, V. P.
AU - Gutierrez, P.
AU - Bhat, D.
AU - Rochelle, J.
AU - Graw, S.
AU - Schalling, M.
AU - Aburatani, H.
AU - Brown, S. D M
AU - Seldin, Michael F
AU - Housman, D. E.
PY - 1994/10
Y1 - 1994/10
N2 - We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.
AB - We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.
UR - http://www.scopus.com/inward/record.url?scp=0028519133&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028519133&partnerID=8YFLogxK
U2 - 10.1007/BF00411453
DO - 10.1007/BF00411453
M3 - Article
C2 - 7849394
AN - SCOPUS:0028519133
VL - 5
SP - 597
EP - 607
JO - Mammalian Genome
JF - Mammalian Genome
SN - 0938-8990
IS - 10
ER -