Rapid 3D fluorescence imaging of individual optically trapped living immune cells

Deanna Wolfson, Michael Steck, Martin Persson, Gregory Mcnerney, Ana Popovich, Mattias Goksör, Thomas R Huser

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We demonstrate an approach to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over the position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging. This system is particularly useful in the handling and investigation of the behavior of non-adherent immune cells. We demonstrate these capabilities by imaging and manipulating living, fluorescently stained Jurkat T cells.

Original languageEnglish (US)
Pages (from-to)208-216
Number of pages9
JournalJournal of Biophotonics
Volume8
Issue number3
DOIs
StatePublished - Mar 1 2015

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Keywords

  • 3-D imaging
  • Confocal microscopy
  • Fluorescence microscopy
  • Jurkat cells
  • Optical tweezers

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wolfson, D., Steck, M., Persson, M., Mcnerney, G., Popovich, A., Goksör, M., & Huser, T. R. (2015). Rapid 3D fluorescence imaging of individual optically trapped living immune cells. Journal of Biophotonics, 8(3), 208-216. https://doi.org/10.1002/jbio.201300153