RAPD markers for constructing intraspecific tomato genetic maps

Majid R. Foolad, Richard A. Jones, Raymond L. Rodriguez

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The existing molecular genetic maps of the tomato, Lycopersicon spp, are constructed based on isozyme and RFLP polymorphisms between tomato species. These maps are useful for certain applications but have few markers that exhibit sufficient polymorphisms for intraspecific analysis and manipulations within the cultivated tomato. The purpose of this study was to investigate the relative potential of RAPD technology, as compared to isozymes and RFLPs, to generate polymorphic DNA markers within cultivated tomatoes. Sixteen isozymes and 25 RFLP clones that were known to detect polymorphism between L. esculentum and L. pennellii, and 313 random oligonucleotide primers were examined. None of the isozymes and only four of the RFLP clones (i.e., 16%) revealed polymorphism between the cultivated varieties whereas up to 63% of the RAPD primers detected one or more polymorphic DNA fragments between these varieties. All RAPD primers detected polymorphism between L. esculentum and L. pennellii genotypes. These results clearly indicate that RAPD technology can generate sufficient genetic markers exploiting sequence differences within cultivated tomatoes to facilitate construction of intraspecific genetic maps.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalPlant Cell Reports
Volume12
Issue number5
DOIs
StatePublished - Mar 1993

Keywords

  • DNA polymorphism
  • Genetic marker
  • Intraspecific genetic map
  • Isozyme
  • Lycopersicon esculentum
  • RAPD
  • RFLP

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'RAPD markers for constructing intraspecific tomato genetic maps'. Together they form a unique fingerprint.

Cite this