Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise

Andrew Philp, Simon Schenk, Joaquin Perez-Schindler, D. Lee Hamilton, Leigh Breen, Erin Laverone, Stewart Jeromson, Stuart M. Phillips, Keith Baar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The present study aimed to investigate the role of the mechanistic target of rapamycin complex 1 (mTORC1) in the regulation of myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis following endurance exercise. Forty-two female C57BL/6 mice performed 1 h of treadmill running (18 m min<sup>-1</sup>; 5° grade), 1 h after i.p. administration of rapamycin (1.5 mg·kg<sup>-1</sup>) or vehicle. To quantify skeletal muscle protein fractional synthesis rates, a flooding dose (50 mg·kg<sup>-1</sup>) of l-[ring-<sup>13</sup>C<inf>6</inf>]phenylalanine was administered via i.p. injection. Blood and gastrocnemius muscle were collected in non-exercised control mice, as well as at 0.5, 3 and 6 h after completing exercise (n = 4 per time point). Skeletal muscle MyoPS and MitoPS were determined by measuring isotope incorporation in their respective protein pools. Activation of the mTORC1-signalling cascade was measured via direct kinase activity assay and immunoblotting, whereas genes related to mitochondrial biogenesis were measured via a quantitative RT-PCR. MyoPS increased rapidly in the vehicle group post-exercise and remained elevated for 6 h, whereas this response was transiently blunted (30 min post-exercise) by rapamycin. By contrast, MitoPS was unaffected by rapamycin, and was increased over the entire post-exercise recovery period in both groups (P < 0.05). Despite rapid increases in both MyoPS and MitoPS, mTORC1 activation was suppressed in both groups post-exercise for the entire 6 h recovery period. Peroxisome proliferator activated receptor-γ coactivator-1α, pyruvate dehydrogenase kinase 4 and mitochondrial transcription factor A mRNA increased post-exercise (P < 0.05) and this response was augmented by rapamycin (P < 0.05). Collectively, these data suggest that endurance exercise stimulates MyoPS and MitoPS in skeletal muscle independently of mTORC1 activation. Journal compilation

Original languageEnglish (US)
Pages (from-to)4275-4284
Number of pages10
JournalJournal of Physiology
Volume593
Issue number18
DOIs
StatePublished - Sep 1 2015

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Mitochondrial Proteins
Sirolimus
Skeletal Muscle
Peroxisome Proliferator-Activated Receptors
Muscle Proteins
Organelle Biogenesis
Phenylalanine
Inbred C57BL Mouse
Immunoblotting
Isotopes
Running
Phosphotransferases
Polymerase Chain Reaction
Messenger RNA
Injections
mechanistic target of rapamycin complex 1
Genes
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Philp, A., Schenk, S., Perez-Schindler, J., Hamilton, D. L., Breen, L., Laverone, E., ... Baar, K. (2015). Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise. Journal of Physiology, 593(18), 4275-4284. https://doi.org/10.1113/JP271219

Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise. / Philp, Andrew; Schenk, Simon; Perez-Schindler, Joaquin; Hamilton, D. Lee; Breen, Leigh; Laverone, Erin; Jeromson, Stewart; Phillips, Stuart M.; Baar, Keith.

In: Journal of Physiology, Vol. 593, No. 18, 01.09.2015, p. 4275-4284.

Research output: Contribution to journalArticle

Philp, A, Schenk, S, Perez-Schindler, J, Hamilton, DL, Breen, L, Laverone, E, Jeromson, S, Phillips, SM & Baar, K 2015, 'Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise', Journal of Physiology, vol. 593, no. 18, pp. 4275-4284. https://doi.org/10.1113/JP271219
Philp, Andrew ; Schenk, Simon ; Perez-Schindler, Joaquin ; Hamilton, D. Lee ; Breen, Leigh ; Laverone, Erin ; Jeromson, Stewart ; Phillips, Stuart M. ; Baar, Keith. / Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise. In: Journal of Physiology. 2015 ; Vol. 593, No. 18. pp. 4275-4284.
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