Randomly amplified polymorphic DNA (RAPD) analysis of Mycoplasma gallisepticum isolates from turkeys from the central valley of California

B. R. Charlton, A. A. Bickford, Richard P Chin, R. L. Walker

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD) analysis was used to investigate the molecular epidemiology of 26 Mycoplasma gallisepticum (MG) isolates obtained from turkeys located in the central valley of California. The MG isolates were recovered from 5 different companies and 13 ranches. Each company had unique MG strains. No evidence of spread of MG between companies was detected. RAPD analysis of MG isolates within a ranch during an outbreak revealed only a single strain involved in each outbreak. RAPD analysis identified an isolate from 1 ranch with a banding pattern identical to that of the 6/85 vaccine strain, which had been used on that particular ranch. Similar RAPD banding patterns of isolates from different ranches within the same company suggested horizontal spread of MG between ranches. The use of 2 primer sets in RAPD analysis was critical to prevent misinterpretation of relationships between different isolates.

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number5
StatePublished - Sep 1999

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Mycoplasma gallisepticum
Central Valley of California
ranching
DNA
Disease Outbreaks
molecular epidemiology
Molecular Epidemiology
Vaccines
vaccines

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Randomly amplified polymorphic DNA (RAPD) analysis of Mycoplasma gallisepticum isolates from turkeys from the central valley of California. / Charlton, B. R.; Bickford, A. A.; Chin, Richard P; Walker, R. L.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 5, 09.1999, p. 408-415.

Research output: Contribution to journalArticle

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