RAmy2A; a novel α-amylase-encoding gene in rice

Ning Huang, Stephen J. Reinl, Raymond L. Rodriguez

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The structure and expression of the α-amylase-encoding gene, RAmy2A, are described. This only representative of the Amy2 subfamily in rice differs from other cereal α-amylase-encoding genes in several respects. It contains the largest introns of all the cereal α-amylase-encoding genes examined to date. Moreover, the second of three introns in this gene contains a long inverted repeat sequence that can potentially form a large and stable stem-loop structure in the unspliced RNA transcript. Finally, RAmy2A is constitutively expressed at very low levels in germinated seeds, root, etiolated leaves, immature seeds and callus. This is in marked contrast to the Amy2 genes of wheat and barley which are highly expressed in the aleurone layer of the germinated seeds.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalGene
Volume111
Issue number2
DOIs
StatePublished - Feb 15 1992

Keywords

  • introns
  • molecular evolution
  • Nucleotide sequence
  • polymerase chain reaction
  • RNA blot

ASJC Scopus subject areas

  • Genetics

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    Huang, N., Reinl, S. J., & Rodriguez, R. L. (1992). RAmy2A; a novel α-amylase-encoding gene in rice. Gene, 111(2), 223-228. https://doi.org/10.1016/0378-1119(92)90690-Q