Abstract
The structure and expression of the α-amylase-encoding gene, RAmy2A, are described. This only representative of the Amy2 subfamily in rice differs from other cereal α-amylase-encoding genes in several respects. It contains the largest introns of all the cereal α-amylase-encoding genes examined to date. Moreover, the second of three introns in this gene contains a long inverted repeat sequence that can potentially form a large and stable stem-loop structure in the unspliced RNA transcript. Finally, RAmy2A is constitutively expressed at very low levels in germinated seeds, root, etiolated leaves, immature seeds and callus. This is in marked contrast to the Amy2 genes of wheat and barley which are highly expressed in the aleurone layer of the germinated seeds.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 223-228 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 111 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 15 1992 |
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Keywords
- introns
- molecular evolution
- Nucleotide sequence
- polymerase chain reaction
- RNA blot
ASJC Scopus subject areas
- Genetics
Cite this
RAmy2A; a novel α-amylase-encoding gene in rice. / Huang, Ning; Reinl, Stephen J.; Rodriguez, Raymond L.
In: Gene, Vol. 111, No. 2, 15.02.1992, p. 223-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RAmy2A; a novel α-amylase-encoding gene in rice
AU - Huang, Ning
AU - Reinl, Stephen J.
AU - Rodriguez, Raymond L.
PY - 1992/2/15
Y1 - 1992/2/15
N2 - The structure and expression of the α-amylase-encoding gene, RAmy2A, are described. This only representative of the Amy2 subfamily in rice differs from other cereal α-amylase-encoding genes in several respects. It contains the largest introns of all the cereal α-amylase-encoding genes examined to date. Moreover, the second of three introns in this gene contains a long inverted repeat sequence that can potentially form a large and stable stem-loop structure in the unspliced RNA transcript. Finally, RAmy2A is constitutively expressed at very low levels in germinated seeds, root, etiolated leaves, immature seeds and callus. This is in marked contrast to the Amy2 genes of wheat and barley which are highly expressed in the aleurone layer of the germinated seeds.
AB - The structure and expression of the α-amylase-encoding gene, RAmy2A, are described. This only representative of the Amy2 subfamily in rice differs from other cereal α-amylase-encoding genes in several respects. It contains the largest introns of all the cereal α-amylase-encoding genes examined to date. Moreover, the second of three introns in this gene contains a long inverted repeat sequence that can potentially form a large and stable stem-loop structure in the unspliced RNA transcript. Finally, RAmy2A is constitutively expressed at very low levels in germinated seeds, root, etiolated leaves, immature seeds and callus. This is in marked contrast to the Amy2 genes of wheat and barley which are highly expressed in the aleurone layer of the germinated seeds.
KW - introns
KW - molecular evolution
KW - Nucleotide sequence
KW - polymerase chain reaction
KW - RNA blot
UR - http://www.scopus.com/inward/record.url?scp=0026581505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026581505&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(92)90690-Q
DO - 10.1016/0378-1119(92)90690-Q
M3 - Article
C2 - 1541400
AN - SCOPUS:0026581505
VL - 111
SP - 223
EP - 228
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -