Raman spectroscopy

Label-free cell analysis and sorting

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Quantitative, label-free biochemical analysis of single, living cells is a highly sought after modality in the biological sciences. While flow cytometry provides a variety of parameters for characterizing and sorting cells, it currently lacks a method for noninvasive and nondestructive chemical analysis. This chapter introduces Raman spectroscopy as a new component in flow cytometry for label-free analysis and sorting of living cells. While similar in principle to current fluorescence-based sorting systems in its design layout and operation, key differences between the Raman and fluorescence process necessitate research focused on developing novel optical and microfluidic schemes applicable for Raman analysis of cells. A particular emphasis must be placed on addressing the weak Raman signal intensity, which is a major obstacle to realizing a Raman sorting system. Although unlikely to rival current cytometers in its throughput, a Raman system could offer new capabilities such as sorting smaller populations for broad biological applications, especiallywhen noninvasive, label-free analysis of living cells is a key requirement. This chapter will introduce the basic principles of Raman spectroscopy, the basic instrumentation, and both seminal and recent studies that have established the foundation for achieving Raman-activated flow cytometry.

Original languageEnglish (US)
Title of host publicationThe Microflow Cytometer
PublisherPan Stanford Publishing Pte. Ltd.
Pages243-261
Number of pages19
ISBN (Print)9789814267410
DOIs
StatePublished - May 1 2010

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Sorting
Raman spectroscopy
Labels
Flow cytometry
Cells
Fluorescence
Microfluidics
Throughput
Chemical analysis

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Chan, J. W. (2010). Raman spectroscopy: Label-free cell analysis and sorting. In The Microflow Cytometer (pp. 243-261). Pan Stanford Publishing Pte. Ltd.. https://doi.org/10.4032/9789814267687

Raman spectroscopy : Label-free cell analysis and sorting. / Chan, James W.

The Microflow Cytometer. Pan Stanford Publishing Pte. Ltd., 2010. p. 243-261.

Research output: Chapter in Book/Report/Conference proceedingChapter

Chan, JW 2010, Raman spectroscopy: Label-free cell analysis and sorting. in The Microflow Cytometer. Pan Stanford Publishing Pte. Ltd., pp. 243-261. https://doi.org/10.4032/9789814267687
Chan JW. Raman spectroscopy: Label-free cell analysis and sorting. In The Microflow Cytometer. Pan Stanford Publishing Pte. Ltd. 2010. p. 243-261 https://doi.org/10.4032/9789814267687
Chan, James W. / Raman spectroscopy : Label-free cell analysis and sorting. The Microflow Cytometer. Pan Stanford Publishing Pte. Ltd., 2010. pp. 243-261
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