Abstract
Raman micro-spectroscopy provides a convenient non-destructive and location-specific means of probing cellular physiology and tissue physiology at sub-micron length scales. By probing the vibrational signature of molecules and molecular groups, the distribution and metabolic products of small molecules that cannot be labeled with fluorescent dyes can be analyzed. This method works well for molecular concentrations in the micro-molar range and has been demonstrated as a valuable tool for monitoring drug-cell interactions. If the small molecule of interest does not contain groups that would allow for a discrimination against cytoplasmic background signals, "labeling" of the molecule by isotope substitution or by incorporating other unique small groups, e.g. alkynes provides a stable signal even for time-lapse imaging such compounds in living cells. In this review we highlight recent progress in assessing the physiology of cells and tissue by Raman spectroscopy and imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 57-70 |
| Number of pages | 14 |
| Journal | Advanced Drug Delivery Reviews |
| Volume | 89 |
| DOIs | |
| State | Published - Jul 15 2015 |
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Keywords
- Cell physiology
- Coherent Raman scattering
- Inelastic light scattering
- Label-free optical microscopy
- Raman scattering
- Single cell analysis
- Tissue physiology
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Raman spectroscopy for physiological investigations of tissues and cells. / Huser, Thomas R; Chan, James W.
In: Advanced Drug Delivery Reviews, Vol. 89, 15.07.2015, p. 57-70.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Raman spectroscopy for physiological investigations of tissues and cells
AU - Huser, Thomas R
AU - Chan, James W
PY - 2015/7/15
Y1 - 2015/7/15
N2 - Raman micro-spectroscopy provides a convenient non-destructive and location-specific means of probing cellular physiology and tissue physiology at sub-micron length scales. By probing the vibrational signature of molecules and molecular groups, the distribution and metabolic products of small molecules that cannot be labeled with fluorescent dyes can be analyzed. This method works well for molecular concentrations in the micro-molar range and has been demonstrated as a valuable tool for monitoring drug-cell interactions. If the small molecule of interest does not contain groups that would allow for a discrimination against cytoplasmic background signals, "labeling" of the molecule by isotope substitution or by incorporating other unique small groups, e.g. alkynes provides a stable signal even for time-lapse imaging such compounds in living cells. In this review we highlight recent progress in assessing the physiology of cells and tissue by Raman spectroscopy and imaging.
AB - Raman micro-spectroscopy provides a convenient non-destructive and location-specific means of probing cellular physiology and tissue physiology at sub-micron length scales. By probing the vibrational signature of molecules and molecular groups, the distribution and metabolic products of small molecules that cannot be labeled with fluorescent dyes can be analyzed. This method works well for molecular concentrations in the micro-molar range and has been demonstrated as a valuable tool for monitoring drug-cell interactions. If the small molecule of interest does not contain groups that would allow for a discrimination against cytoplasmic background signals, "labeling" of the molecule by isotope substitution or by incorporating other unique small groups, e.g. alkynes provides a stable signal even for time-lapse imaging such compounds in living cells. In this review we highlight recent progress in assessing the physiology of cells and tissue by Raman spectroscopy and imaging.
KW - Cell physiology
KW - Coherent Raman scattering
KW - Inelastic light scattering
KW - Label-free optical microscopy
KW - Raman scattering
KW - Single cell analysis
KW - Tissue physiology
UR - http://www.scopus.com/inward/record.url?scp=84938848948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938848948&partnerID=8YFLogxK
U2 - 10.1016/j.addr.2015.06.011
DO - 10.1016/j.addr.2015.06.011
M3 - Article
C2 - 26144996
AN - SCOPUS:84938848948
VL - 89
SP - 57
EP - 70
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
SN - 0169-409X
ER -