Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state

James W Chan, D. Motton, John C Rutledge, N. L. Keim, Thomas R Huser

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Individual triglyceride-rich lipoprotein (TGRL) particles derived from human volunteers are nondestructively analyzed by laser tweezers Raman microspectroscopy, and information on their composition and distribution is obtained. The Raman signature of single optically trapped very low-density lipoproteins (VLDL), a subclass of TGRL, which play an important role in cardiovascular disease, exhibits distinct peaks associated with molecular vibrations of fatty acids, proteins, lipids, and structural rearrangements of lipids. Our analysis of pre- and postprandial VLDL exhibits the signature of biochemical changes in individual lipoprotein particles following the consumption of meals. Interaction of VLDL with endothelium leads to the breakdown of complex triacylglycerols and the formation of a highly ordered core of free saturated fatty acids in the particle. A particle distribution analysis reveals trends in the degree to which this process has occurred in particles at different times during the postprandial period. Differences in particle distributions based on the different ratios of polyunsaturated to saturated fats in the consumed meals are also easily discerned. Individual lipoprotein particles hydrolyzed in vitro through addition of lipoprotein lipase (LpL) exhibit strikingly similar changes in their Raman spectra. These results demonstrate the feasibility of monitoring the dynamics of lipid metabolism of individual TGRL particles as they interact with LpL in the endothelial cell wall using Raman spectroscopy.

Original languageEnglish (US)
Pages (from-to)5870-5876
Number of pages7
JournalAnalytical Chemistry
Volume77
Issue number18
DOIs
StatePublished - Sep 15 2005

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Spectroscopic analysis
Lipoproteins
Triglycerides
VLDL Lipoproteins
Lipoprotein Lipase
Fatty Acids
Molecular vibrations
Lipids
Endothelial cells
Raman spectroscopy
Raman scattering
Fats
Lasers
Monitoring
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. / Chan, James W; Motton, D.; Rutledge, John C; Keim, N. L.; Huser, Thomas R.

In: Analytical Chemistry, Vol. 77, No. 18, 15.09.2005, p. 5870-5876.

Research output: Contribution to journalArticle

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