Rainbow beads: A color coding method to facilitate high-throughput screening and optimization of one-bead one-compound combinatorial libraries

Juntao Luo, Hongyong Zhang, Wenwu Xiao, Pappanaicken R. Kumaresan, Changying Shi, Chong-Xian Pan, Olulanu H. Aina, Kit Lam

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We have developed a new color-encoding method that facilitates high-throughput screening of one-bead one-compound (OBOC) combinatorial libraries. Polymer beads displaying chemical compounds or families of compounds are stained with oil-based organic dyes that are used as coding tags. The color dyes do not affect cell binding to the compounds displayed on the surface of the beads. We have applied such rainbow beads in a multiplex manner to discover and profile ligands against cell surface receptors. In the first application, a series of OBOC libraries with different scaffolds or motifs are each color-coded; small samples of each library are then combined and screened concurrently against live cells for cell attachment. Preferred libraries can be rapidly identified and selected for subsequent large-scale screenings for cell surface binding ligands. In a second application, beads with a series of peptide analogues (e.g., alanine scan) are color-coded, combined, and tested for binding against a specific cell line in a single-tissue culture well; the critical residues required for binding can be easily determined. In a third application, ligands reacting against a series of integrins are color-coded and used as a readily applied research tool to determine the integrin profile of any cell type. One major advantage of this straightforward and yet powerful method is that only an ordinary inverted microscope is needed for the analysis, instead of sophisticated (and expensive) fluorescent microscopes or flow cytometers.

Original languageEnglish (US)
Pages (from-to)599-604
Number of pages6
JournalJournal of Combinatorial Chemistry
Volume10
Issue number4
DOIs
StatePublished - Jul 2008

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Discrete Mathematics and Combinatorics

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