Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro

Heinz Schmidberger, Margret Rave-Fränk, Jörg Lehmann, Simone Schweinfurth, Olivier Pradier, Clemens F. Hess

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: The enhancing effect of natural interferon-β (n-IFN-β) or recombinant interferon-β (r-IFN-β) on radiation damage in tumor cells has been evaluated in several studies. It is not clear whether the different forms of IFN-β available today are equally efficient in modulating the intrinsic radiosensitivity of tumor cells. The purpose of this study was to compare the radiosensitizing effect of n-IFN-β, r-IFN-β1a, and r-IFN-β1b in one cancer cell line. Methods: The A549 lung-cancer cell line was grown as a monolayer culture and incubated for 24 h with n-IFN-β (Fiblaferon), r- IFN-β1a (Betaserin), or r-IFN-β1b (Betaferon). Thereafter, the cultures were irradiated with single graded doses of 0, 1, 2, 4, and 6 Gy. Cellular survival was counted in a colony-forming assay at 10 days after treatment. Survival curves were established using the linear quadratic model. Statistical comparison of the survival data was performed using Student's t- test for each dose point. Interactions of IFN-β and radiation were evaluated using isobologram analysis. Results: All three types of IFN-β enhanced the radiation sensitivity of A549 cells in a similar way as shown in the alteration of the survival curves and the isobologram analysis. The isoeffective concentration of r-IFN-β1b was 2.7-fold higher than that of r- IFN-β1a or n-IFN-β. All three interferons increased the α-component of the survival curves in a concentration-dependent way, suggesting an influence on repair of radiation damage. The maximal sensitizing enhancement ratio (SER) obtained with n-IFN-β or r-IFN-β1a at 3000 IU/ml was 1.66 and 1.51, respectively. The highest SER, obtained with r-IFN-β1b, at 8000 IU/ml was 1.93. Conclusions: All three interferons tested can equally modify the intrinsic radiosensitivity of A549 cells. The isoeffective concentration of r-IFN-β1b is 2.7-fold that of n-IFN-β or r-IFN-β1a.

Original languageEnglish (US)
Pages (from-to)350-356
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume125
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Radiation-Sensitizing Agents
Interferons
Carcinoma
Lung
Radiation Tolerance
Survival
In Vitro Techniques
Radiation
Cell Line
Neoplasms

Keywords

  • Cytokines
  • Interferon
  • Lung cancer
  • Radiosensitization
  • Recombinant beta-interferon Radiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro. / Schmidberger, Heinz; Rave-Fränk, Margret; Lehmann, Jörg; Schweinfurth, Simone; Pradier, Olivier; Hess, Clemens F.

In: Journal of Cancer Research and Clinical Oncology, Vol. 125, No. 6, 1999, p. 350-356.

Research output: Contribution to journalArticle

Schmidberger, H, Rave-Fränk, M, Lehmann, J, Schweinfurth, S, Pradier, O & Hess, CF 1999, 'Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro', Journal of Cancer Research and Clinical Oncology, vol. 125, no. 6, pp. 350-356. https://doi.org/10.1007/s004320050285
Schmidberger, Heinz ; Rave-Fränk, Margret ; Lehmann, Jörg ; Schweinfurth, Simone ; Pradier, Olivier ; Hess, Clemens F. / Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro. In: Journal of Cancer Research and Clinical Oncology. 1999 ; Vol. 125, No. 6. pp. 350-356.
@article{778128171b2f4d378fbfaae3ef45c4b2,
title = "Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro",
abstract = "Purpose: The enhancing effect of natural interferon-β (n-IFN-β) or recombinant interferon-β (r-IFN-β) on radiation damage in tumor cells has been evaluated in several studies. It is not clear whether the different forms of IFN-β available today are equally efficient in modulating the intrinsic radiosensitivity of tumor cells. The purpose of this study was to compare the radiosensitizing effect of n-IFN-β, r-IFN-β1a, and r-IFN-β1b in one cancer cell line. Methods: The A549 lung-cancer cell line was grown as a monolayer culture and incubated for 24 h with n-IFN-β (Fiblaferon), r- IFN-β1a (Betaserin), or r-IFN-β1b (Betaferon). Thereafter, the cultures were irradiated with single graded doses of 0, 1, 2, 4, and 6 Gy. Cellular survival was counted in a colony-forming assay at 10 days after treatment. Survival curves were established using the linear quadratic model. Statistical comparison of the survival data was performed using Student's t- test for each dose point. Interactions of IFN-β and radiation were evaluated using isobologram analysis. Results: All three types of IFN-β enhanced the radiation sensitivity of A549 cells in a similar way as shown in the alteration of the survival curves and the isobologram analysis. The isoeffective concentration of r-IFN-β1b was 2.7-fold higher than that of r- IFN-β1a or n-IFN-β. All three interferons increased the α-component of the survival curves in a concentration-dependent way, suggesting an influence on repair of radiation damage. The maximal sensitizing enhancement ratio (SER) obtained with n-IFN-β or r-IFN-β1a at 3000 IU/ml was 1.66 and 1.51, respectively. The highest SER, obtained with r-IFN-β1b, at 8000 IU/ml was 1.93. Conclusions: All three interferons tested can equally modify the intrinsic radiosensitivity of A549 cells. The isoeffective concentration of r-IFN-β1b is 2.7-fold that of n-IFN-β or r-IFN-β1a.",
keywords = "Cytokines, Interferon, Lung cancer, Radiosensitization, Recombinant beta-interferon Radiation",
author = "Heinz Schmidberger and Margret Rave-Fr{\"a}nk and J{\"o}rg Lehmann and Simone Schweinfurth and Olivier Pradier and Hess, {Clemens F.}",
year = "1999",
doi = "10.1007/s004320050285",
language = "English (US)",
volume = "125",
pages = "350--356",
journal = "Journal of Cancer Research and Clinical Oncology",
issn = "0171-5216",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Radiosensitizing effect of natural and recombinant β-interferons in a human lung carcinoma in vitro

AU - Schmidberger, Heinz

AU - Rave-Fränk, Margret

AU - Lehmann, Jörg

AU - Schweinfurth, Simone

AU - Pradier, Olivier

AU - Hess, Clemens F.

PY - 1999

Y1 - 1999

N2 - Purpose: The enhancing effect of natural interferon-β (n-IFN-β) or recombinant interferon-β (r-IFN-β) on radiation damage in tumor cells has been evaluated in several studies. It is not clear whether the different forms of IFN-β available today are equally efficient in modulating the intrinsic radiosensitivity of tumor cells. The purpose of this study was to compare the radiosensitizing effect of n-IFN-β, r-IFN-β1a, and r-IFN-β1b in one cancer cell line. Methods: The A549 lung-cancer cell line was grown as a monolayer culture and incubated for 24 h with n-IFN-β (Fiblaferon), r- IFN-β1a (Betaserin), or r-IFN-β1b (Betaferon). Thereafter, the cultures were irradiated with single graded doses of 0, 1, 2, 4, and 6 Gy. Cellular survival was counted in a colony-forming assay at 10 days after treatment. Survival curves were established using the linear quadratic model. Statistical comparison of the survival data was performed using Student's t- test for each dose point. Interactions of IFN-β and radiation were evaluated using isobologram analysis. Results: All three types of IFN-β enhanced the radiation sensitivity of A549 cells in a similar way as shown in the alteration of the survival curves and the isobologram analysis. The isoeffective concentration of r-IFN-β1b was 2.7-fold higher than that of r- IFN-β1a or n-IFN-β. All three interferons increased the α-component of the survival curves in a concentration-dependent way, suggesting an influence on repair of radiation damage. The maximal sensitizing enhancement ratio (SER) obtained with n-IFN-β or r-IFN-β1a at 3000 IU/ml was 1.66 and 1.51, respectively. The highest SER, obtained with r-IFN-β1b, at 8000 IU/ml was 1.93. Conclusions: All three interferons tested can equally modify the intrinsic radiosensitivity of A549 cells. The isoeffective concentration of r-IFN-β1b is 2.7-fold that of n-IFN-β or r-IFN-β1a.

AB - Purpose: The enhancing effect of natural interferon-β (n-IFN-β) or recombinant interferon-β (r-IFN-β) on radiation damage in tumor cells has been evaluated in several studies. It is not clear whether the different forms of IFN-β available today are equally efficient in modulating the intrinsic radiosensitivity of tumor cells. The purpose of this study was to compare the radiosensitizing effect of n-IFN-β, r-IFN-β1a, and r-IFN-β1b in one cancer cell line. Methods: The A549 lung-cancer cell line was grown as a monolayer culture and incubated for 24 h with n-IFN-β (Fiblaferon), r- IFN-β1a (Betaserin), or r-IFN-β1b (Betaferon). Thereafter, the cultures were irradiated with single graded doses of 0, 1, 2, 4, and 6 Gy. Cellular survival was counted in a colony-forming assay at 10 days after treatment. Survival curves were established using the linear quadratic model. Statistical comparison of the survival data was performed using Student's t- test for each dose point. Interactions of IFN-β and radiation were evaluated using isobologram analysis. Results: All three types of IFN-β enhanced the radiation sensitivity of A549 cells in a similar way as shown in the alteration of the survival curves and the isobologram analysis. The isoeffective concentration of r-IFN-β1b was 2.7-fold higher than that of r- IFN-β1a or n-IFN-β. All three interferons increased the α-component of the survival curves in a concentration-dependent way, suggesting an influence on repair of radiation damage. The maximal sensitizing enhancement ratio (SER) obtained with n-IFN-β or r-IFN-β1a at 3000 IU/ml was 1.66 and 1.51, respectively. The highest SER, obtained with r-IFN-β1b, at 8000 IU/ml was 1.93. Conclusions: All three interferons tested can equally modify the intrinsic radiosensitivity of A549 cells. The isoeffective concentration of r-IFN-β1b is 2.7-fold that of n-IFN-β or r-IFN-β1a.

KW - Cytokines

KW - Interferon

KW - Lung cancer

KW - Radiosensitization

KW - Recombinant beta-interferon Radiation

UR - http://www.scopus.com/inward/record.url?scp=0033010156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033010156&partnerID=8YFLogxK

U2 - 10.1007/s004320050285

DO - 10.1007/s004320050285

M3 - Article

C2 - 10363567

AN - SCOPUS:0033010156

VL - 125

SP - 350

EP - 356

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

SN - 0171-5216

IS - 6

ER -