Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111

S. L. Mills, S. J. De Nardo, Gerald L Denardo, S. V. Deshpande, M. McCall, C. Meares, A. L. Epstein

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

(S)-4-[2,3 bis[bis(carboxymethylamino)propyl]phenyl isothio-cyanate (CITC) was used to attach In-111 to an intact monoclonal antibody (Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90% immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per mg F(Ab')2. The labeling reaction produced yields in excess of 98% eliminating the requirement for routine purification of the final radiopharmaceutical.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume27
Issue number4
StatePublished - 1989

Fingerprint

Cyanates
Radiopharmaceuticals
Lymphoma
Imaging techniques
Molecules
B-Cell Lymphoma
Labeling
Purification
Cells
Antibodies
Lym-1 monoclonal antibody

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111. / Mills, S. L.; De Nardo, S. J.; Denardo, Gerald L; Deshpande, S. V.; McCall, M.; Meares, C.; Epstein, A. L.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 27, No. 4, 1989, p. 377-386.

Research output: Contribution to journalArticle

Mills, S. L. ; De Nardo, S. J. ; Denardo, Gerald L ; Deshpande, S. V. ; McCall, M. ; Meares, C. ; Epstein, A. L. / Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111. In: Journal of Labelled Compounds and Radiopharmaceuticals. 1989 ; Vol. 27, No. 4. pp. 377-386.
@article{5e655d6716174be29bac88e9753f273b,
title = "Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111",
abstract = "(S)-4-[2,3 bis[bis(carboxymethylamino)propyl]phenyl isothio-cyanate (CITC) was used to attach In-111 to an intact monoclonal antibody (Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90{\%} immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per mg F(Ab')2. The labeling reaction produced yields in excess of 98{\%} eliminating the requirement for routine purification of the final radiopharmaceutical.",
author = "Mills, {S. L.} and {De Nardo}, {S. J.} and Denardo, {Gerald L} and Deshpande, {S. V.} and M. McCall and C. Meares and Epstein, {A. L.}",
year = "1989",
language = "English (US)",
volume = "27",
pages = "377--386",
journal = "Journal of Labelled Compounds and Radiopharmaceuticals",
issn = "0362-4803",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111

AU - Mills, S. L.

AU - De Nardo, S. J.

AU - Denardo, Gerald L

AU - Deshpande, S. V.

AU - McCall, M.

AU - Meares, C.

AU - Epstein, A. L.

PY - 1989

Y1 - 1989

N2 - (S)-4-[2,3 bis[bis(carboxymethylamino)propyl]phenyl isothio-cyanate (CITC) was used to attach In-111 to an intact monoclonal antibody (Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90% immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per mg F(Ab')2. The labeling reaction produced yields in excess of 98% eliminating the requirement for routine purification of the final radiopharmaceutical.

AB - (S)-4-[2,3 bis[bis(carboxymethylamino)propyl]phenyl isothio-cyanate (CITC) was used to attach In-111 to an intact monoclonal antibody (Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90% immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per mg F(Ab')2. The labeling reaction produced yields in excess of 98% eliminating the requirement for routine purification of the final radiopharmaceutical.

UR - http://www.scopus.com/inward/record.url?scp=0024519911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024519911&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0024519911

VL - 27

SP - 377

EP - 386

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

SN - 0362-4803

IS - 4

ER -