Abstract
(S)-4-[2,3 bis[bis(carboxymethylamino)propyl]phenyl isothio-cyanate (CITC) was used to attach In-111 to an intact monoclonal antibody (Lym-1) or its fragment F(ab')2 to produce two new radiopharmaceuticals. Immunoreactivity of the Lym-1, specific for B cell lymphoma, was not affected by the coupling reaction for 1.0 and 4.3 CITC molecules per whole antibody and 3.0 CITC molecules per F(ab')2 fragment. Both retained in excess of 90% immunoreactivity even at the level of 25 mCi of In-111 per mg of Lym-1 and 15 mCi per mg F(Ab')2. The labeling reaction produced yields in excess of 98% eliminating the requirement for routine purification of the final radiopharmaceutical.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 377-386 |
| Number of pages | 10 |
| Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
| Volume | 27 |
| Issue number | 4 |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine
- Analytical Chemistry
- Organic Chemistry
- Drug Discovery
- Pharmacology
Cite this
Mills, S. L., De Nardo, S. J., Denardo, G. L., Deshpande, S. V., McCall, M., Meares, C., & Epstein, A. L. (1989). Radiopharmaceutical preparation of a monoclonal antibody, Lym-1, and its F(ab')2 fragment for imaging lymphoma with In-111. Journal of Labelled Compounds and Radiopharmaceuticals, 27(4), 377-386.