Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water

K. E. Wainwright, M. Lagunas-Solar, M. A. Miller, B. C. Barr, A. C. Melli, A. E. Packham, N. Zeng, T. Truong, Patricia A Conrad

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Summary Toxoplasma gondii, a ubiquitous parasitic protozoan, is emerging as an aquatic biological pollutant. Infections can result from drinking water contaminated with environmentally resistant oocysts. However, recommendations regarding water treatment for oocyst inactivation have not been established. In this study, the physical method of radiofrequency (RF) power was evaluated for its ability to inactivate T. gondii oocysts in water. Oocysts were exposed to various RF energy levels to induce 50, 55, 60, 70 and 80°C temperatures maintained for 1 min. Post-treatment oocyst viability was determined by mouse bioassay with serology, immunohistochemistry and in vitro parasite isolation to confirm T. gondii infections in mice. None of the mice inoculated with oocysts treated with RF-induced temperatures of ≥60°C in an initial experiment became infected; however, there was incomplete oocyst activation in subsequent experiments conducted under similar conditions. These results indicate that T. gondii oocysts may not always be inactivated when exposed to a minimum of 60°C for 1 min. The impact of factors such as water heating time, cooling time and the volume of water treated must be considered when evaluating the efficacy of RF power for oocyst inactivation.

Original languageEnglish (US)
Pages (from-to)74-81
Number of pages8
JournalZoonoses and Public Health
Volume57
Issue number1
DOIs
StatePublished - Feb 2010

Fingerprint

Oocysts
heat inactivation
Toxoplasma
Toxoplasma gondii
oocysts
Hot Temperature
Water
water
mice
inactivation
Temperature
Water Purification
Toxoplasmosis
Serology
water treatment
Drinking Water
infection
Biological Assay
Heating
drinking water

Keywords

  • Oocyst
  • Radiofrequency
  • Toxoplasma gondii
  • Water treatment

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Epidemiology
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Wainwright, K. E., Lagunas-Solar, M., Miller, M. A., Barr, B. C., Melli, A. C., Packham, A. E., ... Conrad, P. A. (2010). Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water. Zoonoses and Public Health, 57(1), 74-81. https://doi.org/10.1111/j.1863-2378.2009.01280.x

Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water. / Wainwright, K. E.; Lagunas-Solar, M.; Miller, M. A.; Barr, B. C.; Melli, A. C.; Packham, A. E.; Zeng, N.; Truong, T.; Conrad, Patricia A.

In: Zoonoses and Public Health, Vol. 57, No. 1, 02.2010, p. 74-81.

Research output: Contribution to journalArticle

Wainwright, KE, Lagunas-Solar, M, Miller, MA, Barr, BC, Melli, AC, Packham, AE, Zeng, N, Truong, T & Conrad, PA 2010, 'Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water', Zoonoses and Public Health, vol. 57, no. 1, pp. 74-81. https://doi.org/10.1111/j.1863-2378.2009.01280.x
Wainwright KE, Lagunas-Solar M, Miller MA, Barr BC, Melli AC, Packham AE et al. Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water. Zoonoses and Public Health. 2010 Feb;57(1):74-81. https://doi.org/10.1111/j.1863-2378.2009.01280.x
Wainwright, K. E. ; Lagunas-Solar, M. ; Miller, M. A. ; Barr, B. C. ; Melli, A. C. ; Packham, A. E. ; Zeng, N. ; Truong, T. ; Conrad, Patricia A. / Radiofrequency-induced thermal inactivation of toxoplasma gondii oocysts in water. In: Zoonoses and Public Health. 2010 ; Vol. 57, No. 1. pp. 74-81.
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