Radiation-sterilized insoluble collagenous bone matrix is a functional carrier of osteogenin for bone induction

Ronald W. Katz, Greg C. Felthousen, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The influence of gamma radiation on the role of the collagenous substratum as a carrier for proteins which cause bone induction was examined. Osteoinductive demineralized bone matrix was extracted by 4 M guanidinium hydrochloride. The insoluble collagenous bone matrix (ICBM) obtained was not osteoinductive; however, when reconstituted with partially purified osteogenin, bone induction was restored. In order to apply the principle of bone induction to clinical use, methods of sterilization must be optimized to maintain the osteoinductive activity of bone allografts. The inactive substratum was irradiated and reconstituted with an active, partially purified bone extract and bioassayed. Irradiation of the ICBM by a Cobalt 60 source at a dose of 1 and 3 Mrads had no deleterious effect on the functional role of the substratum.

Original languageEnglish (US)
Pages (from-to)183-185
Number of pages3
JournalCalcified Tissue International
Issue number3
StatePublished - Sep 1990
Externally publishedYes


  • Alkaline Phosphatase
  • Collagenous Substratum
  • Osteogenin
  • Radiation

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism


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