Radiation-force assisted targeting facilitates ultrasonic molecular imaging

Shukui Zhao, Mark Borden, Susannah H. Bloch, Dustin Kruse, Katherine W. Ferrara, Paul A. Dayton

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Ultrasonic molecular imaging employs contrast agents, such as microbubbles, nanoparticles, or liposomes, coated with ligands specific for receptors expressed on cells at sites of angiogenesis, inflammation, or thrombus. Concentration of these highly echogenic contrast agents at a target site enhances the ultrasound signal received from that site, promoting ultrasonic detection and analysis of disease states. In this article, we show that acoustic radiation force can be used to displace targeted contrast agents to a vessel wall, greatly increasing the number of agents binding to available surface receptors. We provide a theoretical evaluation of the magnitude of acoustic radiation force and show that it is possible to displace micron-sized agents physiologically relevant distances. Following this, we show in a series of experiments that acoustic radiation force can enhance the binding of targeted agents: The number of biotinylated microbubbles adherent to a synthetic vessel coated with avidin increases as much as 20-fold when acoustic radiation force is applied; the adhesion of contrast agents targeted to α vβ 3 expressed on human umbilical vein endothelial cells increases 27-fold within a mimetic vessel when radiation force is applied; and finally, the image signal-to-noise ratio in a phantom vessel increases up to 25 dB using a combination of radiation force and a targeted contrast agent, over use of a targeted contrast agent alone.

Original languageEnglish (US)
Pages (from-to)135-148
Number of pages14
JournalMolecular Imaging
Volume3
Issue number3
DOIs
StatePublished - Jul 2004

Fingerprint

Ultrasonic imaging
Molecular imaging
Molecular Imaging
Contrast Media
Ultrasonography
Radiation
Acoustics
Microbubbles
Avidin
Human Umbilical Vein Endothelial Cells
Signal-To-Noise Ratio
Ultrasonics
Liposomes
Nanoparticles
Thrombosis
Endothelial cells
Ligands
Inflammation
Signal to noise ratio
Adhesion

Keywords

  • Bjerknes force
  • Contrast agent
  • Microbubble
  • Molecular imaging
  • Radiation force
  • Targeted imaging
  • Ultrasound

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Zhao, S., Borden, M., Bloch, S. H., Kruse, D., Ferrara, K. W., & Dayton, P. A. (2004). Radiation-force assisted targeting facilitates ultrasonic molecular imaging. Molecular Imaging, 3(3), 135-148. https://doi.org/10.1162/1535350042380317

Radiation-force assisted targeting facilitates ultrasonic molecular imaging. / Zhao, Shukui; Borden, Mark; Bloch, Susannah H.; Kruse, Dustin; Ferrara, Katherine W.; Dayton, Paul A.

In: Molecular Imaging, Vol. 3, No. 3, 07.2004, p. 135-148.

Research output: Contribution to journalArticle

Zhao, S, Borden, M, Bloch, SH, Kruse, D, Ferrara, KW & Dayton, PA 2004, 'Radiation-force assisted targeting facilitates ultrasonic molecular imaging', Molecular Imaging, vol. 3, no. 3, pp. 135-148. https://doi.org/10.1162/1535350042380317
Zhao, Shukui ; Borden, Mark ; Bloch, Susannah H. ; Kruse, Dustin ; Ferrara, Katherine W. ; Dayton, Paul A. / Radiation-force assisted targeting facilitates ultrasonic molecular imaging. In: Molecular Imaging. 2004 ; Vol. 3, No. 3. pp. 135-148.
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