RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange

Erica M. Seitz, Joel P. Brockman, Steven J. Sandler, A. John Clark, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

With the discovery that the Saccharomyces cerevisiae Rad51 protein is both structurally and functionally similar to the Escherichia coli RecA protein, the RecA paradigm for homologous recombination was extended to the Eucarya. The ubiquitous presence of RecA and Rad51 protein homologs raises the question of whether this archetypal protein exists within the third domain of life, the Archaea. Here we present the isolation of a Rad51/RecA protein homolog from the archaeon Sulfolobus solfataricus, and show that this protein, RadA, possesses the characteristics of a DNA strand exchange protein: The RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange.

Original languageEnglish (US)
Pages (from-to)1248-1253
Number of pages6
JournalGenes and Development
Volume12
Issue number9
StatePublished - May 1 1998

Fingerprint

Archaeal Proteins
Rec A Recombinases
DNA
Archaea
Proteins
Sulfolobus solfataricus
Saccharomyces cerevisiae Proteins
Nucleoproteins
Escherichia coli Proteins
Homologous Recombination
Eukaryota
Adenosine Triphosphatases

Keywords

  • Archaea
  • DNA strand exchange
  • RadA
  • RecA
  • Recombination

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Seitz, E. M., Brockman, J. P., Sandler, S. J., Clark, A. J., & Kowalczykowski, S. C. (1998). RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes and Development, 12(9), 1248-1253.

RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. / Seitz, Erica M.; Brockman, Joel P.; Sandler, Steven J.; Clark, A. John; Kowalczykowski, Stephen C.

In: Genes and Development, Vol. 12, No. 9, 01.05.1998, p. 1248-1253.

Research output: Contribution to journalArticle

Seitz, EM, Brockman, JP, Sandler, SJ, Clark, AJ & Kowalczykowski, SC 1998, 'RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange', Genes and Development, vol. 12, no. 9, pp. 1248-1253.
Seitz EM, Brockman JP, Sandler SJ, Clark AJ, Kowalczykowski SC. RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes and Development. 1998 May 1;12(9):1248-1253.
Seitz, Erica M. ; Brockman, Joel P. ; Sandler, Steven J. ; Clark, A. John ; Kowalczykowski, Stephen C. / RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. In: Genes and Development. 1998 ; Vol. 12, No. 9. pp. 1248-1253.
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