Rad54 Functions as a Heteroduplex DNA Pump Modulated by Its DNA Substrates and Rad51 during D Loop Formation

William Douglass Wright, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The displacement loop (D loop) is the product of homology search and DNA strand invasion, constituting a central intermediate in homologous recombination (HR). In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation by the Rad54 motor protein. Curiously, Rad54 also disrupts D loops. How these opposing activities are coordinated toward productive recombination is unknown. Moreover, a seemingly disparate function of Rad54 is removal of Rad51 from heteroduplex DNA (hDNA) to allow HR-associated DNA synthesis. Here, we uncover features of D loop formation/dissociation dynamics, employing Rad51 filaments formed on ssDNAs that mimic the physiological length and structure of invivo substrates. The Rad54 motor is activated by Rad51 bound to synapsed DNAs and guided by a ssDNA-binding domain. We present a unified model wherein Rad54 acts as an hDNA pump that drives D loop formation while simultaneously removing Rad51 from hDNA, consolidating both ATP-dependent activities of Rad54 into a single mechanistic step.

Original languageEnglish (US)
Pages (from-to)420-432
Number of pages13
JournalMolecular Cell
Volume53
Issue number3
DOIs
StatePublished - Feb 6 2014

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Nucleic Acid Heteroduplexes
Homologous Recombination
DNA
Eukaryota
Genetic Recombination
Proteins
Adenosine Triphosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Rad54 Functions as a Heteroduplex DNA Pump Modulated by Its DNA Substrates and Rad51 during D Loop Formation. / Wright, William Douglass; Heyer, Wolf Dietrich.

In: Molecular Cell, Vol. 53, No. 3, 06.02.2014, p. 420-432.

Research output: Contribution to journalArticle

Wright, William Douglass ; Heyer, Wolf Dietrich. / Rad54 Functions as a Heteroduplex DNA Pump Modulated by Its DNA Substrates and Rad51 during D Loop Formation. In: Molecular Cell. 2014 ; Vol. 53, No. 3. pp. 420-432.
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