Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation

Tomohiko Sugiyama, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

The Rad51 nucleoprotein filament mediates DNA strand exchange, a key step of homologous recombination. This activity is stimulated by replication protein A (RPA), but only when RPA is introduced after Rad51 nucleoprotein filament formation. In contrast, RPA inhibits Rad51 nucleoprotein complex formation by prior binding to single-stranded DNA (ssDNA), but Rad52 protein alleviates this inhibition. Here we show that Rad51 filament formation is simultaneous with displacement of RPA from ssDNA. This displacement is initiated by a rate-limiting nucleation of Rad51 protein onto ssDNA complex, followed by rapid elongation of the filament. Rad52 protein accelerates RPA displacement by Rad51 protein. This acceleration probably involves direct interactions with both Rad51 protein and RPA. Detection of a Rad52-RPA-ssDNA co-complex suggests that this co-complex is an intermediate in the displacement process.

Original languageEnglish (US)
Pages (from-to)31663-31672
Number of pages10
JournalJournal of Biological Chemistry
Volume277
Issue number35
DOIs
StatePublished - Aug 30 2002

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Rad52 DNA Repair and Recombination Protein
Replication Protein A
Single-Stranded DNA
Nucleoproteins
Proteins
Homologous Recombination
Elongation
Nucleation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation. / Sugiyama, Tomohiko; Kowalczykowski, Stephen C.

In: Journal of Biological Chemistry, Vol. 277, No. 35, 30.08.2002, p. 31663-31672.

Research output: Contribution to journalArticle

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