Rabbit heart fatty acid-binding protein: Isolation, characterization, and application of a monoclonal antibody

Anne A Knowlton, R. E. Burrier, P. Brecher

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 ± 9 μg/ml) compared with patients with pulmonary edema (52 ± 7 μg/ml) and normal volunteers (28 ± 5 μg/ml; p < 0.05, mean ± SEM).

Original languageEnglish (US)
Pages (from-to)981-988
Number of pages8
JournalCirculation Research
Volume65
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Fatty Acid-Binding Proteins
Monoclonal Antibodies
Rabbits
Proteins
Isoelectric Point
Pulmonary Edema
Tissue Distribution
Diaphragm
Affinity Chromatography
Immunoassay
Aorta
Healthy Volunteers
Fatty Acids
Myocardial Infarction
Amino Acids
Muscles
Antibodies
human FABP3 protein

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Rabbit heart fatty acid-binding protein : Isolation, characterization, and application of a monoclonal antibody. / Knowlton, Anne A; Burrier, R. E.; Brecher, P.

In: Circulation Research, Vol. 65, No. 4, 1989, p. 981-988.

Research output: Contribution to journalArticle

@article{870a587a26a344a49f46712227fd5a69,
title = "Rabbit heart fatty acid-binding protein: Isolation, characterization, and application of a monoclonal antibody",
abstract = "A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 ± 9 μg/ml) compared with patients with pulmonary edema (52 ± 7 μg/ml) and normal volunteers (28 ± 5 μg/ml; p < 0.05, mean ± SEM).",
author = "Knowlton, {Anne A} and Burrier, {R. E.} and P. Brecher",
year = "1989",
language = "English (US)",
volume = "65",
pages = "981--988",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Rabbit heart fatty acid-binding protein

T2 - Isolation, characterization, and application of a monoclonal antibody

AU - Knowlton, Anne A

AU - Burrier, R. E.

AU - Brecher, P.

PY - 1989

Y1 - 1989

N2 - A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 ± 9 μg/ml) compared with patients with pulmonary edema (52 ± 7 μg/ml) and normal volunteers (28 ± 5 μg/ml; p < 0.05, mean ± SEM).

AB - A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 ± 9 μg/ml) compared with patients with pulmonary edema (52 ± 7 μg/ml) and normal volunteers (28 ± 5 μg/ml; p < 0.05, mean ± SEM).

UR - http://www.scopus.com/inward/record.url?scp=0024468734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024468734&partnerID=8YFLogxK

M3 - Article

C2 - 2791232

AN - SCOPUS:0024468734

VL - 65

SP - 981

EP - 988

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 4

ER -